Biomimetic surface modification of titanium implants

目的：本研究的目的是开发一种新的仿生表面修饰的钛种植体磷酸化氨基酸，并评价其对成骨细胞MC 3 T3-E1的初始附着的有效性。方法在室温下分别用10 N盐酸（HCl）和超纯水对工业纯钛（cp Ti盘直径：5.8 mm，厚度：2 mm ;机械加工表面Ra：0.15 μ m JIS 2 ; GC Co，Tokyo，Japan）超声处理30 min。然后，这些磁盘修饰O-磷酸-L-苏氨酸（P-Thr）在37 ℃下12小时，然后用超纯水超声冲洗10分钟。未处理的磁盘作为对照。X射线光电子能谱（XPS）化学分析的P-Thr改性的磁盘表面与对照相比。另外，通过10、30、60 min时的贴壁细胞百分比来评价成骨细胞MC 3 T3-E1在每个改性表面和对照表面上的初始贴壁。结果如下：XPS分析表明，经HCl和超纯水处理后，P-Thr的P2p结合能发生了显著的化学位移（t检验：P < 0.05）。在10分钟时，细胞附着到P-Thr修饰的表面的百分比显著高于对照的百分比（单因素ANOVA和Bonferroni/Dunn检验：P <0.01）。结论：结果表明，经盐酸和超纯水处理的纯钛表面存在P-Thr的化学结合，P-Thr修饰的纯钛表面能促进成骨细胞的早期附着

Purpose : The purpose of this stud was to develop a new biomimetic surface modification of titanium implat with phospho-amino acid and evaluate its effectiveness on initial attachment of osteoblasts MC3T3-E1. Method Commercially pure titanium (cp Ti disks diameter : 5.8 mm thickness : 2 mm ; machined surface Ra : 0.15 micron JIS 2 ; GC Co, Tokyo, Japan) were ultrasonically treated with 10 N hydrochloric acid (HCl) and ultrapure water at room temperature for 30 min respectively. Then these disks were modified with O-phospho-L-threonine (P-Thr) at 37 C degree far 12 hrs followed ultrasonic rinsing with ultrapure water for 10 min. Untreated disks were used as a control. X-ray hotoelectron spectroscopy (XPS) chemically analyzed the P-Thr-modified disk surfaces in comparison with the control. In addition initial attachment of osteoblasts MC3T3-E1 to each of modifed surfaces and the control was evaluated by the percentage of attached cells at 10 30 60 min respectively. Results : XPS revealed that P-Thr chemically bonded to the surface treated with HCl and ultrapure water since the significant chemical shift (t-test : P < 0.05) of P 2p binding energy of P-Thr was detected. Percentage at 10 min of cellular attachment to the P-Thr-modified surface was significantly higher than that of the control (one-way ANOV and Bonferroni/Dunn's test : P < 0,01). Conclusion : It is concluded that chemical banding of P-Thr to the cp Ti surface treated with HCl and ultrapure water was detected and the P-Thr-modified surface can accelerate the initial osteoblasts attachment