IMPROVEMET AND DEVELOPMENT FOR IMPLANT MATERIALS USING HUMAN GINGIVAL EPITHELIAL CELLS

1991年，石川从人牙龈建立了人上皮细胞系，并将其命名为HGE-15。由于我们已经使用HGE-15细胞研究了各种生物材料的生物相容性。2002年至2003年的结果如下：2002年，SEM拍摄了HGE-15细胞在Falcon、MRT和CT上孵育30分钟的照片。大多数HGE-15细胞贴壁生长，可见丝状伪足和片状伪足，约占30%。2 h后，F和MRT上HGE细胞形态发生改变，呈多角形扩张，具有上皮细胞的特征，其比例高于CT。24小时后，样品的整个表面被多边形细胞覆盖。通过TEM在F、MRT和CT上孵育24小时后，在材料和细胞之间未观察到半桥粒。而在MRT、CT和F附近的细胞表面则零星可见包被小泡和包被小凹。在基质旁边观察到厚度约为25 nm的双层结构 ...更多信息 钛和猎鹰的表面都是椭圆形的。但我们没有观察到明确的细胞外基质的存在。然而，1周后，观察到无定形密度层，这被认为表明细胞外基质的存在。在MRT上，未观察到双层结构，仅在细胞侧显示（图3）。这一发现仅在MRT上得到认可。我们的研究结果表明，从人牙龈获得的HGE-15细胞具有最高的MRT亲和力。我们从TEM结果得出结论，1周后细胞外基质粘附在材料侧。2003年，我们使用人正常成骨细胞进行了研究，以比较其他细胞。结果如下：30分钟：大多数NHOst细胞粘附、改变形式并扩增。在F、MPT和HTT上观察到丝状伪足和片状伪足。未发现材料之间存在明显差异。1小时：比较LM上细胞团的形态学特征。与MPT相比，清楚地观察到细胞的铺展和增殖。此外，个体细胞结构中最显著的变化与HTT相关。与F和MPT相比，细胞在外周区域以细长的薄图案延伸。2小时后，这种趋势变得更加显著。结果表明，在NHOst细胞的铺展和增强方面，HTT处理的水热处理比F和MPT处理的水热处理具有上级优势，材料的差异在于Ca离子的存在。结论：它是骨细胞生长的重要因素。通过两年来的研究结果，得出结论：对生物材料进行化学改性是可行的。少

In 1991, Ishikawa established a human epithelial cell line from human gingiva and named it HGE-15. Since we have studied the biocompatibility for various biomaterials using HGE-15 cells. The results of this from 2002 to 2003 are as followsIn 2002, SEM photograph 30-minute-incubation of HGE-15 cells on Falcon, MRT, and CT. Most of HGE-15 cells adhered on MRT and observed the Filopodian and Lamellipodia were observed about 30%. After 2 hours, many of the HGE-cells were changed the form and expanded with polygonal shape having characteristic of epithelial cells on F and MRT, and its rate were more than CT. After 24 hours, the whole surface of the sample was covered with the polygonal cells. No hemidesmosome was seen between the materials and cells 24-hour-incubation on F, MRT, and CT by TEM. However, coated vesicles and coated pits were observed sporadically on the surface of cells adjacent to MRT, CT, and F. A double-layered structure of about 25nm in thickness was seen next to the mater … More ial surface on Ti and Falcon. But we did not observe the existence of the clear extracellar matrix. However, 1 week after, the amorphous density layer was observed which is thought to indicate the existence of extracellular matrix. On MRT, a double-layered structure was not seen and was shown only on the cellular side (Figure 3). This finding was recognized only on MRT. Our results indicate that HGE-15 cells obtained from human gingiva have the highest affinty for MRT. We conclude that the extracellular matrix adhered to the material side from TEM findings after 1 week.In 2003, we have carried out the studies using Human Normal Osteoblast cells in order to compare the other cell. Results as follows ; 30 minutes : Most of the NHOst cells adhered, changed form, and expanded. Filopodia and lamellipodia were observed on F, MPT, and HTT. No clear difference between the materials was recognized. 1 hour : compares the morphologic characteristics of the cell mass on LM. Spreading and proliferation of cells were clearly observed as compared with MPT. Furthermore, the most remarkable changes in individual cell structure were associated with HTT. Cells extended in an elongated thin pattern as compared with F and MPT at peripheral regions. After 2 hours, this tendency became more remarkable. As the results, we have concluded that HTT treated hydrothermal was superior to F ad MPT regarding spreading and enhancement for NHOst cells.The difference of materials is the present of Ca ion. We have concluded that it is important factor for osteos cells. The result from two years, I conclude that the giving us one suggestion due to carry out the chemical modification of biomaterials. Less