Gene expression of Oral Leukoplakia and Early Squamous Cell Carcinoma DNA Array Study

应用基因芯片技术研究口腔白斑、早期鳞状细胞癌、腺样囊性癌及其癌旁正常组织的基因表达谱。对每个标本的mRNA表达水平进行多变量分析。探索性建模分析使用递归搜索树确定了31个基因，这些基因是信息丰富的，有助于区分肿瘤病变和正常健康组织。这些基因的功能并不是一个统一的状态，而是表现出多种特性，如肿瘤促进因子、肿瘤抑制因子、细胞骨架因子、细胞因子、细胞粘附因子等，并采用Ward方法对基因表达谱进行了系统聚类分析，结果表明，每种肿瘤病变的基因表达与原始正常组织的相似性高于另一种肿瘤。在树状图中，首先将检测样本分为粘膜组织来源和唾液腺来源2大类。其次，将每个主要聚类区分为正常组织和肿瘤性病变。鳞状细胞癌和白斑病组织样本显示了非常相似的基因表达模式。腺样囊性癌与正常涎腺相邻。一簇正常唾液腺组织位于离一簇鳞状细胞癌和白斑最远的位置

Gene expression profile of oral leukoplakia, early squamous cell carcinoma, adenoid cystic carcinoma and their neighboring normal tissues were demonstrated by using DNA array method. Multivariate analyses for mRNA expression levels of each specimen were done. Exploratory modeling analysis using recursive search tree determined 31genes that are informative and helpful to discriminate tumorous lesions from normal healthy tissue. The functions of these genes are not a uniform state, but showed a variety of characters as tumor accelerators, tumor suppressors, cytoskeletons, cytokines, cell adhesion factors, and so on.Hierarchical cluster analysis of gene expression profile using ward method was also carried out and resulted as follows ; gene expression of each tumorous lesion have higher similarity to that of original normal tissue than that of another kind of tumors. In the tree diagram, examined samples were firstly divided into 2 major clusters as mucosal tissue origin and salivary gland origin. In the next place, each major cluster was discriminated normal tissue from tumorous lesion. Tissue samples of squamous cell carcinomas and leukoplakias showed closely same gene expression pattern. Adenoid cystic carcinoma and normal salivary gland made neighboring clusters. A cluster of normal salivary gland tissue was located in the farthest site from a cluster of squamous cell carcinomas and leukoplakias