Development of the gene deletion model rats by random mutagenesis.

用苯丁酸氮芥随机诱变，以产生一些新的突变大鼠。为了确定苯丁酸氮芥的最佳剂量和诱变的最佳交配时间，我们计数了用几个剂量的苯丁酸氮芥给药的雌性大鼠与雄性大鼠交配所生的新生大鼠的数量。因此，很明显，每公斤体重2.5毫克至15毫克的苯丁酸氮芥是良好的药物治疗，并进行交配后的苯丁酸氮芥的药物治疗后三周至六周是好的。我们从BN和F344品系获得了几只侏儒大鼠。还观察到体型约为正常大鼠30%的侏儒大鼠。我们还从BN系获得了一只具有结肠肉瘤的突变大鼠。这个淋巴肉瘤在两周内已经生长到直径5cm或更大。遗憾的是，我们还没有通过定量PCR分析确定该大鼠的基因组缺失区域。未来的实验将集中于通过微阵列或BAC阵列分析来搜索该基因组缺失区域。

Random mutagenesis with chlorambucil was performed in order to produce some new mutant rats. To identify the optimal quantity of chlorambucil and the optimal mating time for mutagenesis, we counted the number of new born rats born from female rats mated with the male rat medicated with several dose of chlorambucil. Consequently, it became clear that between 2.5mg and 15mg per kg weight of chlorambucil are good for medication, and it is good to perform mating between three weeks and six weeks after the medication of chlorambucil. We obtained several dwarfish rats from BN and F344 strans. Dwarfish rats whose sizes of the body were about 30% of normal rats were also observed. We also obtained a mutant rat with fiblosarcoma from the BN strain. This fiblosarcoma has grown up to 5cm or more in the diameter within 2 weeks. Unfortunately, we have not determined the genome region of delition of this rat by quontitative PCR analysis. Future experiments will focus on searching this genome region of delition by microarray or BAC array analyses.