Molecular diversity of polyphenolic compounds in fruits and leaves and these new physiological functions

在这项研究中，我们已经开发了一个系统的高效液相色谱分析多酚化合物的分子量和分子量分布与各种分子种类，这是从各种样品制备; roouibos茶，枇杷叶和幼果，柿子叶和幼果，番石榴茶叶。该系统已非常有用，简单地表征多酚从他们的分子大小和MW分布。将来自这些样品的纯化级分应用于每个生物测定。结果表明，从路易波士茶的馏分在抗菌试验中显示出高活性。柿叶单宁具有较高的诱导癌细胞程序性死亡活性，其中柿幼果单宁的分子量最大，分子量分布最窄。因此，我们认为这种独特的聚合物可能是工业用途的良好材料。我们研究了高分子链、单宁分子之间的各种连接反应，因此，我们认为这种独特的聚合物可能是一种良好的工业用途材料。我们研究了高分子链与单宁分子之间的各种连接反应，并发现了两种单宁分子交联剂，以产生一种新的凝胶或树脂。所述凝胶或树脂可用于制造生物可食用产品和生物可降解生活商品。本论文主要包括三个部分：（1）建立高效液相色谱条件，快速分析分子量和分子量分布，以及聚合多酚的降解;（2）研究多酚的新生理功能;（3）探索单宁分子间交联的新方法，制备新的凝胶和树脂。

In this study, we have developed a system of HPLC to analyze the molecular weight and the MW distribution of polyphenolic compounds with various molecular species, which were prepared from various samples; roouibos tea, loquat leaf and young fruit, persimmon leaf and young fruit, guava tea leaf. The system has been very useful to characterize simply the polyphenols from them as to molecular size and MW distribution. The purified fractions from these samples were applied to each bio-assays. As a result, a fraction from rooibos tea showed a high activity in the antibacterial assay. A fraction from persimmon leaf indicated a high activity of inducing programmed cell death of cancer cells.It has been evident that a purified tannin prepared from persimmon young fruit has Most higheast molecular size and most narrow distribution of MW among those which were examined in this study. So, we have supposed that this unique polymer may be a good material for industrial usages. We have investigated various reactions of linkages between highly polymeric chains, tannin molecules So, we have supposed that this unique polymer may be a good material for industrial usages. We have investigated various reactions of linkages between highly polymeric chains, tannin molecules and found two crosslinking agents of tannin molecules to produce a new gel or a resin. The gel or resin may be available to make bio-eatable products and bio-degradative living commodities. This report is composed of three parts;(1) Establishment of HPLC conditions to analyze MW and MW distribution with higher speed and degradation of polymeric polyphenols(2) Investigating new physiological functions of polyphenols(3) Finding a new method of crosslinking among tannin moleculesto produce a new gel and resin.