Investigation of protein secreted from cultured cell line in insect

从昆虫细胞系NIAS-AeA1-2的条件培养液中分离纯化了乙酰胆碱酯酶。在SES-NABR-4和SES-NABR-93细胞中检测到昆虫EGF类生长因子，在NIAS-AeA1-2和SES-MaBR-4细胞中检测到胰蛋白酶抑制物。我试图纯化该蛋白，但在亲和柱层析步骤中，该蛋白与胰酶紧密吸附。该蛋白的相对分子质量和功能尚不清楚。RT-PCR检测SES-NABR-4细胞中的多巴脱羧酶

Acetylcholinesterase was purified from conditioned medium of insect cell line NIAS-AeA1-2. SDS-PAGE was suggested that molecular weight was 67kDa, and gel-filtrated chromatography suggested this protein was dimmer in conditioned medium.Growth blocking peptide, an insect EGF type growth factor was detected by RT-PCR in SES-NaBr-4 and SES-NaBr-93 cell line.Trypsin inhibitor was detected in NIAS-AeA1-2, and SES-MaBr-4. I tried to purify this protein but in step of affinity column chromatography, this protein adsorbed tightly to trypsin. This protein still unclear about molecular weight and function.Dopa-decalboxilase was detected by RT-PCR in SES-NaBr-4 Cell line