Establishment of general procedure to synthesize polysialogangliosides and its application for neurobidogy.

鞘糖脂是真核细胞特有的膜成分，与其他鞘糖脂不同，它含有一个或多个唾液酸残基，在许多生物过程中，特别是在神经系统中发挥着重要作用。研究成果分为以下两部分。高效构建聚硅寡糖。α(2-4)-和α(2-8)双二酸序列采用1,5-内酰胺化的唾液酸受体高产得到。得到的二糖被用于构建唾液寡糖。寡糖与神经酰胺的分子内糖基传递偶联。将神经酰胺拴在葡萄糖上，用琥珀酸还原低聚糖末端，使低聚糖和神经酰胺有效偶联。得到的葡萄糖基神经酰胺与唾液寡糖作为构建块进一步糖基化，得到所需的神经节苷。

Glycosphingolipids are characteristic membrane components of eukaryotic cells where they are distinguished from other glycosphingolipids by containing one or more sialic acid residues, and have been recognized play important roles in many biological processes, particularly in nervous system We have succeeded in the establishment of general procedunes to synthesize polysialogangliosides. The achievements are summarized in the following two parts.1.Efficient construction of polysialo-oligosaccharides.α(2-4)- and α(2-8)disialic acid seguences were obtained in high yields by employing 1,5-lactamized sialyl acceptors. The disaccharides obtained were utilized for the construction of sialo-oligosaccharides.2.The coupling of oligosaccharides and ceramides by intramolecular-aglycon derivery.Tethering of ceramide to glucose, reducing terminal of oligosaccharides, with succnic acid allowed the efficient coupling of oligosaccharides and ceramides. The glucosyl ceramide obtained was further glycosylated with sialo-oligosaccharides as building bloks to give the desired gangliosides.