Does the bovine lactoferrin improve chronic inflammation in the immuno-suppressive condition induced by FIV infection?

1)口服牛乳铁蛋白（bLF）对fiv感染猫淋巴细胞功能的影响研究了bLF对cona诱导的健康猫和fiv感染猫外周血淋巴细胞增殖的影响。口服bLF对健康猫和FIV感染猫的淋巴细胞增殖均有抑制作用。bLF和外周淋巴细胞共孵育也显示出对健康猫和感染fiv的猫的淋巴细胞增殖的抑制作用。2) bLF和ConA对猫外周淋巴细胞细胞因子mRNA表达的影响。ConA与外周淋巴细胞共孵育后，采用RT-PCR方法检测IFN-γ、IL-1β、TNF-α和IL-12p40 mRNA表达。在加入ConA后4hr，首次检测到IFN-γ mrna的强表达，但加入ConA后，其他细胞因子如IL-1(3)、TNF-α、IL-12p40等mrna的表达并未增加。采用实时荧光定量PCR检测IFN-γ mrna的表达，以验证RT-PCR的结果。建立了实时PCR检测猫IFN-γ的方法。在ConA刺激后，采用real - time PCR方法在健康猫和fiv感染猫的外周血淋巴细胞中观察到IFN-γ的强mrna表达。在ConA刺激前和ConA刺激后10、20和40 min，将bLF加入外周血淋巴细胞，采用real - time PCR检测ConA诱导的IFN-γ mRNA表达量。牛LF抑制cona诱导的felme淋巴细胞中IFN-γ mrna的表达。淋巴细胞在ConA刺激前30 min及刺激后10、20、40 min均可抑制IFN-γ- mrna的表达。3) ConA刺激对淋巴细胞IFN-γ- mrna表达信号通路的影响探讨ConA刺激与淋巴细胞信号通路的关系。我们的结果表明，ptk和erk依赖的信号通路参与了cona诱导的IFN-γ-mRNA的表达。因此，bLF可能通过干扰这一信号通路抑制IFN-γ的产生。从这些结果来看，bLF在体内的有益作用可能与bLF对猫淋巴细胞的调节能力有关。bLF的这些调节作用似乎与慢性炎症猫的抗炎作用有关。少

1)The effects of orally-administered bovine lactoferrin (bLF) on lymphocyte functions in FIV-infected catsThe effects of bLF on ConA-induced proliferation of peripheral lymphocyte in healthy and FIV-infected cats were investigated. Orally-administered bLF showed the inhibition of lymphocyte proliferation both in healthy and FIV- infected cats. Co-incubation of bLF and peripheral lymphocytes also showed the inhibition of lymphocyte proliferation both in healthy and FIV-infected cats.2)The effects of bLF and ConA on cytokine mRNA expression in feline peripheral lymphocyteAfter the co-incubation of ConA and peripheral lymphocyte, mRNA expression of IFN-γ,IL-1β,TNF-α and IL-12p40 was investigated by RT-PCR method. The strong mRNA-expression of IFN-γ was first detected at 4hr after the addition of ConA, however the addition of ConA did not bring the mRNA-expression of other cytokines such as IL-1(3,TNF-α and IL-12p40. After that, the mRNA-expression of IFN-γ was investigated by quantitative … More analysis with real time PCR in order to confirm the result of RT-PCR. We established the method of real time PCR for feline IFN-γ. After the stimulation of ConA, the strong mRNA-expression of IFN-γ was observed in peripheral lymphocyte both of healthy and FIV-infected cats, using real time PCR method. Before ConA stimulation and 10, 20 and 40 min after ConA stimulation, bLF was added to peripheral lymphocyte and ConA-induced mRNA- expression of IFN-γ was determined by real time PCR. Bovine LF inhibited the ConA-induced mRNA-expression of IFN-γ in felime lymphocyte. The inhibition of IFN-γ-mRNA-expression was observed not only when lymphocyte was treated with bLF 30 min before but also 10,20 or 40 min after ConA stimulation.3)The effects of Con A stimulation on the lymphocyte signaling pathways of IFN-γ-mRNA-expressionThe relation of ConA stimulation and signaling pathways of lymphocyte was investigated. Our results suggested that PTK-and ERK-dependent signaling pathways are involved in the ConA-induced expression of IFN-γ-mRNA. Therefore, it is likely that bLF inhibits IFN-γ production by interfering with this signaling pathway.Judging from these results, the beneficial effects of bLF in vivo may be explained by modulatory ability of bLF against feline lymphocyte. These modulatory actions of bLF seemed to be involved in anti-inflammatory effects on the cats with chronic inflammation. Less