Analysis of relationship between proteins and external stimuli on bone formation/resorption

在硬骨鱼中，钙代谢是通过鳞片而不是骨骼来调节的，特别是在需要钙快速动员的情况下。本研究的第一个目的是确定鳞片是否可以作为研究哺乳动物成骨细胞和破骨细胞之间关系的模型。在鳞片的未暴露部分，ALP阳性细胞，即成骨细胞，位于底部的第一至第三脊之间的板层中，超过第三脊的细胞显示核-核连接，没有ALP和TRAP活性。在暴露部分，共定位的ALP阳性和TRAP阳性细胞（破骨细胞）观察到在外侧周边，表明扩大向横向方向受到严格管制。TRAP阳性细胞在陷窝处被诱导。在未暴露的部分再生鳞片，TRAP活性增强的侧周边，并积极与骨细胞小管的形状在邻近ALP阳性细胞的板层。另一方面，侧线 ...更多信息 管被ALP阳性和TRAP阳性细胞包围。保持侧线管壁很薄是将环境水的运动引入侧线神经末梢的必要条件。探讨侧线管与神经功能的关系是研究周围感觉神经发育的良好模型。我们还发现，为了维持侧线管，notch、凋亡诱导因子以及凋亡抑制因子等都上调。除了古罗马人，这些完整的序列还不为人所知，所以我们克隆了这些信号。此外，我们证实，这些ALP阳性和TRAP阳性细胞表现出与哺乳动物破骨细胞/成骨细胞相似的激素反应。为了预防骨质疏松症，我们一直试图利用VitK 2。我们发现Vit K2抑制哺乳动物成骨细胞的增殖并促进其向骨细胞的分化。ALP阳性细胞对Vit K2的反应相似。因此，ALP和TRAP阳性细胞对外界刺激的反应与哺乳动物成骨细胞/破骨细胞相似。硬骨鱼鳞片是研究成骨细胞/骨细胞/破骨细胞间/细胞内关系的良好模型。少

In teleostei, Ca metabolism is regulated via scale rather than bone, especially in case that rapid mobilization of Ca is required. The first porpose of this study is to determine whether scale can be a model for the research exploring relationship between mammalian osteoblast and osteoclast. In unexposed parts of scale, ALP-positive cells, viz osteoblasts, were located in lamellas between the first to third ridge at the bottom, and cells beyond the third ridge showed nuclei-nuclei linkage without ALP- and TRAP-activity. In exposed parts, co-localization of ALP-positive and TRAP-positive cells (osteoclasts) were observed at the lateral periphery, indicating that enlargement toward the lateral direction was strictly regulated. TRAP-positive cells were induced at pits. In unexposed parts of regenerating scales, TRAP-activity was enhanced at the lateral periphery and was positive with an osteocytic canaliculi shape in lamellas adjacent to ALP-positive cells. On the other hand, lateral line … More tube was surrounded both by ALP-positive and TRAP-positive cells. To maintain the wall of lateral line tube very thin is essential to introduce movement of environmental water to lateral line nerve terminal. It is also considered that to explore how lateral line tube and nerve function is a good model for exploring development of peripheral sensory nerve. We also found that, for the maintenace of lateral line tube, notch, inducers of apoptosis, as well as inhibitors of apoptosis, etc. were up-regulated. Aside from mammalians, these full sequence was yet known, so that we cloned these signals. Furthermore, we confirmed that these ALP-positive and TRAP-positive cells showed response to hormones similar with mammalian osteoclasts/osteoblasts. For the prevention of osteopososis, we have been trying to utilize VitK2. We found that Vit K2 inhibit proliferating mammalian osteoblasts and promote differentiating into osteocyte.s. Scale ALP-positive cells showed similar response to Vit K2. Thus,Both ALP- and TRAP-positive cells displayed similarity to mammalian osteoblast/osteoclast in response to externalstimuli. Teleost scales are transparent to be a good model for studying osteoblast/osteocyte/osteoclast inter/intracellular relationship in vivo and/or as whole mount culture. Less