A basic research on coupling factors between bone resorption and bone formation
A basic research on coupling factors between bone resorption and bone formation
批准号:
16592072
负责人:
HARA Yoshitaka
金额:
$2.24万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2004
资助国家:
日本
项目状态:
已结题
起止时间:
2004 至 2005
中文摘要
RANKL和OPG在脂多糖诱导的骨吸收中的作用尚不清楚。因此,在第一项研究中,我们检测了重复注射脂多糖增加骨吸收,而额外注射PBS减少骨吸收时RANKL和OPG表达细胞数量的变化。随着骨吸收的增强,表达RANKL的炎性细胞增多,表达OPG的非炎性细胞减少。另一方面,表达OPG的炎症细胞数量随着骨吸收的减少而增加。在炎性细胞检测中,骨吸收增加的小鼠皮损中检测到RANKL表达的T细胞,骨吸收减少的小鼠皮损中检测到表达OPG的中性粒细胞。这些结果提示T细胞和PMN在炎症性骨吸收的调节中起着重要作用。第二项研究的目的是探讨B细胞是否真的影响体内的炎症性骨吸收。对正常小鼠、B细胞和T细胞缺失的SCID小鼠和B细胞重组的SCID小鼠牙槽骨吸收进行了组织病理学比较。此外,我们还检测了脂多糖刺激的B细胞是否参与了体外破骨细胞的形成。结果显示,B细胞重组SCID鼠组较SCID鼠组表现出更强的炎性骨吸收。此外,在体外,脂多糖刺激的B细胞促进破骨细胞的生成,抗肿瘤坏死因子-α抗体完全阻断内毒素刺激的B细胞诱导的破骨细胞生成。提示B细胞通过肿瘤坏死因子-α促进炎性骨吸收。
英文摘要
The involvement of RANKL and OPG in lipopolysaccharide-induced bone resorption is not well understood. So in the first study, we examined changes in the number of RANKL- and OPG-expressing cells when bone resorption was increased by repeated injections of LPS and decreased by additional injection of PBS. The number of RANKL-expressing inflammatory cells increased and OPG-expressing non-inflammatory cells decreased with enhancement of bone resorption. On the other hand, the number of OPG-expressing inflammatory cells increased with decrease of bone resorption. Of the inflammatory cells assay, RANKL-expressing T cells were detected in the lesions of mice with increased bone resorption and OPG-expressing polymorphonuclear leukocytes (PMN) were detected in the lesions of mice with decreased bone resorption. These results suggested that T cells and PMNs play important roles in the regulation of inflammatory bone resorption.The aim of the second study was to examine whether B cells truly influence inflammatory bone resorption in vivo. Alveolar bone resorption in normal mice, in SCID mice that lack both B and T cells, and in B cell-reconstituted SCID mice were compared histopathologically after repeated injections of lipopolysaccharide into mouse gingival. Furthermore, we examined whether the B cells that are stimulated by lipopolysaccharide are involved in osteoclastogenesis in vitro. As a result, the B cell-reconstituted SCID mice group showed stronger inflammatory bone resorption than the SCID mice group. Also, in vitro, lipopolysaccharide-stimulated B cells enhanced osteoclastogenesis and anti-TNF-α antibody completely blocked osteoclastogenesis induced by lipopolysaccharide-stimulated B cells. These results suggest that B cells promote inflammatory bone resorption through TNF-α.
期刊论文(10)
专著(0)
科研奖励(0)
会议论文
Immunohistological study on expression of receptor activator of NF-κ B ligand and osteoprotegerin in lipopolysaccharide-induced bone resorption
脂多糖诱导骨吸收中NF-κB配体受体激活剂和骨保护素表达的免疫组织学研究
DOI:
--
发表时间:
2005
期刊:
Journal of Japanese Conservative Dentistry. 48(2)
影响因子:
--
作者:
[Mayumi Yoshimoto, Takashi Ukai, Yoshitaka Hara]
通讯作者:
Yoshitaka Hara
LPS誘導型骨吸収におけるRANKLおよびOPGに関する免疫組織学的研究.
RANKL 和 OPG 在 LPS 诱导骨吸收中的免疫组织学研究。
DOI:
--
发表时间:
2005
期刊:
日本歯科保存学会雑誌 48巻2号
影响因子:
--
作者:
[吉本真弓, 鵜飼 孝, 原 宣興]
通讯作者:
原 宣興
B cells play an important role in LPS-induced bone resorption
B细胞在LPS诱导的骨吸收中发挥重要作用
DOI:
--
发表时间:
2006
期刊:
Calcified Tissue International (In press)
影响因子:
--
作者:
[Kozuka Y, et al.]
通讯作者:
et al.
Immunomodulating blood purification system for sepsis
-
批准号:17K17071
-
项目类别:Grant-in-Aid for Young Scientists (B)
-
资助金额:$2.41万
-
财政年份:2017
-
负责人:HARA Yoshitaka
-
依托单位:
Experimental pathological study on prevention of periodontitis and periimplantitis caused by drugs
-
批准号:15K11395
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$3.08万
-
财政年份:2015
-
负责人:HARA Yoshitaka
-
依托单位:
A study on acceleration of bone resorption by immunized cells
-
批准号:19592389
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$2.91万
-
财政年份:2007
-
负责人:HARA Yoshitaka
-
依托单位:
Immunohistlogical study of mouse periodontal burst model
-
批准号:13672192
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$2.3万
-
财政年份:2001
-
负责人:HARA Yoshitaka
-
依托单位:
Function of cementblast: I vitro study and histopathological study
-
批准号:10671970
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$1.73万
-
财政年份:1998
-
负责人:HARA Yoshitaka
-
依托单位:
A study for establishment of judging periodontal disease activity
-
批准号:08672199
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$1.47万
-
财政年份:1996
-
负责人:HARA Yoshitaka
-
依托单位:
Experimental study of the effect of T cell on alveolar bone resorption induced by bacterial endotoxin
-
批准号:05454519
-
项目类别:Grant-in-Aid for General Scientific Research (B)
-
资助金额:$4.54万
-
财政年份:1993
-
负责人:HARA Yoshitaka
-
依托单位:
Immunoelectron Microscopic Study on Cytoskelcton of Pocket Epithelium and Infiltrating Cells
-
批准号:01571047
-
项目类别:Grant-in-Aid for General Scientific Research (C)
-
资助金额:$1.34万
-
财政年份:1989
-
负责人:HARA Yoshitaka
-
依托单位:
An experimental study on histopathological changes of alveolar bone around hydroxyapatite during orthodontic tooth movement.
-
批准号:62570858
-
项目类别:Grant-in-Aid for General Scientific Research (C)
-
资助金额:$1.34万
-
财政年份:1987
-
负责人:HARA Yoshitaka
-
依托单位:
海外基金