Novel Technologies for the Purification of Recombinant Proteins

获得大规模纯化重组蛋白的技术是生物技术工业的一项基本要求。本计画旨在整合分子生物辨识中的两个概念。固定化金属离子亲和色谱的新模式将与新型肽亲和手柄的选择相关联，所述肽亲和手柄可以通过基因工程方法插入到通过生物技术程序产生的蛋白质中。然后，所遵循的方法允许在分离靶蛋白后去除这些柄。因此，该项目可能导致用于人类治疗的重组蛋白质的制造取得相当大的进展。

Availability of enabling technologies for the large-scale purification of recombinant proteins is an essential requirement of the biotechnology industry. This project aims to integrate two concepts in molecular biorecognition. New modalities of immobilised metal ion affinity chromatography will be linked with the selection of new types of peptide affinity handles that can be inserted by genetic engineering methods into proteins produced by biotechnological procedures. The approach followed then allows these handles to be removed following isolation of the target protein. This project could thus lead to a considerable advance in the manufacture of recombinant proteins intended for human therapy.