Preparation of molecularly imprinted polymer sensor for blood level measurement of intravenous anesthetic.

本研究的目的是研制一种用于异丙酚等静脉麻醉剂血药浓度测量的传感器。采用分子印迹聚合物(MIP)作为异丙酚的敏感层，用石英晶体微天平(QCM)测定丙泊酚的浓度。2004年，在玻片玻璃表面进行了丙泊酚选择性MIP的聚合膜制备。丙泊酚(模板)、甲基丙烯酸(MAA，功能单体)、乙二醇二甲基丙烯酸酯(EGDMA，交联剂)和2，2‘-偶氮双(2.4-二甲基戊腈)(引发剂)的摩尔比分别为1：6.5：8.5：0.4(摩尔比)，溶解于正己烷中。将混合溶液薄涂在载玻片表面，在40℃下聚合48h。将分子印迹聚合物制备成平整、薄层是非常困难的。2005年，我们对MIP膜的制备进行了改进。将MIP聚合方法改为沉淀聚合，制得MIP微球。用聚氯乙烯将MIP微球结合到载玻片玻璃或石英晶体上。然而，将MIP微球制成平层/薄层仍然是困难的。因此，无法估计MIP-QCM系统的性能。还需要进一步的改进

The purpose of this research was preparation of a sensor for blood level measurement of intravenous anesthetic such as propofol. The molecularly imprinted polymers (MIPs) were employed as a propofol sensitive layer, and quartz crystal microbalances (QCM) were employed to determine the propofol concentration.In 2004, for practice of membrane preparation, the propofol selective MIPs were polymerized on the surface of slide glasses. Propofol (template), methacrylic acid (MAA, functional monomer), ethylene glyacol dimethacrylate (EGDMA, cross-linker) and 2,2'-Azobis(2.4-dimethyl valeronitrile) (initiator) were measured 1 : 6.5 : 8.5 : 0.4 (mol ratio, respectively) and dissolved in hexane. The mixture solution was painted thinly on the surface of slide glass and polymerized at 40℃ for 48 hours. It was very difficult to make the MIP into flat and thin layer. In 2005, we tried to improve the MIP layer preparation. The method of MIP polymerization was changed to precipitation polymerization to make MIP into microspheres. The MIP microspheres were binded onto slide glass or quartz crystal with PVC. However, it was still difficult to make MIP microspheres into flat/thin layer. Therefor, the property of MIP-QCM system could not be estimated. Additional improvement was needed