Elucidation of receptors for salivary protein histatin in human gingival fibroblast

牙周病引起口腔细菌接触牙齿和口腔组织。结果显示牙槽骨破坏。这些是由炎症宿主细胞之间复杂的相互作用产生的。我们报道了人牙龈成纤维细胞（HGF）在脂多糖（LPS）或牙周细菌蛋白酶刺激下的组织破坏和炎性细胞因子的产生。组胺素的功能是杀龋齿活性和抑制龋齿细菌增殖。并发现艾滋病组织蛋白表达减少。我们阐明了组蛋白对牙周细菌蛋白酶的灭活作用和对HGF增殖的促进作用。当研究组蛋白在口腔组织中的定位时，我们可以预期它们的功能。用抗组胺素抗体对口腔组织进行染色，腮腺组织中存在组胺素， ...更多信息 下颌下腺、舌颗粒层和颊粘膜刺细胞层。组胺素在腮腺和下颌下腺特异性表达。组胺素5（Histatin 5）是组胺素家族的一员，它与白色念珠菌热休克蛋白（HSP）SSA 1/2p结合。结果表明，该菌具有一定的抑菌作用。然后，我们研究了histatin是否与人类热休克蛋白相互作用。HSC70是HSPs的成员，与HSC70的肽结合结构域结合的组胺素结合。histatin通过内吞作用进入细胞，histatin/HSC 70复合物转位到细胞核内。由于这在细胞的热处理中是显著的，因此表明组胺素的易位依赖于HSC 70。热休克蛋白的功能是蛋白质的结构形成、变性蛋白质的重折叠和防止蛋白质缩合。HSC 70是Toll样受体的配体，与DNA的复制和转录有关。提示HGF的增殖和存活与histatin有关。组蛋白和细胞因子之间的相互作用尚未报道。此外，对histatin和HSP之间的生理功能的详细研究似乎与histatin的药物设计发现有关。这可以应用于口腔疾病和生殖领域。少

Periodontal diseases cause oral bacteria that contact with teeth and oral tissues. The results indicate the destruction of alveolar bones. These are generated by the complicated interaction between inflammatory host cells. We reported that the tissue destruction and production of inflammatory cytokines from human gingival fibroblast (HGF) were stimulated by LPS or protease derived from periodontal bacteria. Functions of histatins are a Candidacidal activity and a suppression of bacterial proliferation for caries. And it found the reduced expression of histatins for AIDS. We clarified about inactivation of proteases from periodontal bacteria and enhancement of proliferation in HGF by histatins. When the localization of histatins in a mouth organization was investigated, we may be expected the functions of those. And the identification of molecles that interact with histatins was investigated.When oral tissues were stained with anti-histatin antibody, histatins were existed in a parotid, … More a submandibular gland, tongue stratum granulosum and buccal mucosa prickle-cell layer. Histatins were specifically expressed in the parotid and the submandibular gland. And histatins may be transported into intercellular or intracellular space of tissues and cells.Histatin 5, a member of histatin family, bound to heat shock protein (HSP) SSA 1/2p from Candida albicans. The result showed antimicrobial action. Then, we investigated whether histatin was interacted with human HSPs. HSC70 that is a member of HSPs was associated with histatin, which bound to the peptide-binding domain of HSC70. Histatin was transported into the cells by the endocytosis and histatin/HSC70 complex was translocated into nuclei. Since this was remarkable at heat treatment of cells, it was suggested that the translocation of histatin is dependent on HSC70. The functions of HSPs are structural formation of proteins, refolding of denatured proteins and prevention of condensational proteins. And HSC70, in relation to a DNA replication and transcription, serves as a ligand of Toll-like receptor. These results suggested that the proliferation and survival of HGF was related with histatin. The interaction between histatins and cellular factors has not yet been reported. Moreover, it seems that detailed study for the physiological functions between histatin and HSP is connected with drug design discovery for histatin. And this could apply to the oral diseases and employ a field of reproduction. Less