Development and establishment of high performance functional monomers

温和的自酸蚀粘合剂仅部分使牙本质脱矿，在亚微米混合层内的胶原蛋白周围留下羟基磷灰石。这种残留的羟基磷灰石可以作为受体与功能单体的化学相互作用，并随后有助于粘合剂的性能，除了微机械杂交。因此，我们采用X射线光电子能谱（XPS）和原子吸收分光光度计（Atomic Absorption Spectrometry，AES）对三种功能单体与人工合成羟基磷灰石的化学结合作用进行了表征，并采用透射电镜（TEM）对三种功能单体与牙本质的超微形态进行了表征。羟基磷灰石上碳浓度的定量测定揭示了10-MDP与羟基磷灰石的结合潜力明显强于4-MET，即使在暴露30分钟之后。此外，即使羟基磷灰石暴露于10-MDP短的30秒也会产生显著的C1 s峰，并且峰强度不会因延长暴露时间而进一步增加，强烈表明，10-MDP在临床合理的应用时间内具有与羟基磷灰石高的化学键合潜力。2. TEM显示，所有三种自蚀刻粘合剂均形成浅的（0.5-1μm）杂化层。未染色的非脱矿切片显示，所有系统仅部分脱矿牙本质，在亚微米混合层内的胶原蛋白周围留下羟基磷灰石。染色揭示了一个典型的混合层的超微结构与交叉带状胶原分离的电子透明的原纤维间的空间。典型的“粗毛地毯”经常出现在混合层的顶部。10-MDP的钙盐（10-MDPCa）在水中的溶解度最低

Mild self-etch adhesives only partially demineralize dentin, leaving hydroxyapatite around collagen within a submicron hybrid layer. This residual hydroxyapatite may serve as receptor for chemical interaction with the functional monomer and subsequently contribute to adhesive performance in addition to micro-mechanical hybridization. We therefore characterized the adhesive interaction of three functional monomers chemically with synthetic hydroxyapatite using X-ray Photoelectron Spectroscopy and Atomic Absorption Spectrophotometry, and ultra-morphologically with dentin using Transmission Electron Microscopy.1.Comparing chemical bonding efficacy of the three functional monomers investigated, quantitative determination of carbon concentration on hydroxyapatite revealed that the bonding potential of 10-MDP to hydroxyapatite is significantly stronger than that of 4-MET, even after 30-min exposure. Moreover, the fact that even a short 30-s exposure of hydroxyapatite to 10-MDP produced a significant C 1s peak and that the peak intensity increased no further by extending the exposure time, strongly suggests that 10-MDP has a high chemical bonding potential to hydroxyapatite within a clinically reasonable application time.2.TEM revealed that all three self-etch adhesives formed a shallow (0.5-1μm) hybrid layer. Unstained, non-demineralized sections revealed that all systems demineralized dentin only partially, leaving hydroxyapatite around collagen within the submicron hybrid layer. Staining disclosed a typical hybrid-layer ultra-structure with cross-banded collagen separated by electron lucent interfibrillar spaces. A typical ‘shag-carpet' often appeared at the hybrid-layer top. Stained, demineralized sections confirmed the acid-resistance of the hybrid layer.3.The calcium salt of 10-MDP (10-MDPCa) was significantly the least soluble in water