Effects of chemotherapy and angiogenesis inhibitor on invasion and metastasis of oral squamous cell carcinoma

采用可复制口腔癌侵袭和转移的原位种植模型，研究抗癌药物对口腔癌侵袭和转移的抑制作用。将高侵袭性、高转移性的人口腔鳞状细胞癌细胞系OSC-19移植到裸鼠口底，移植后7天或14天分别给予顺铂或培普霉素。研究不同抗癌药物和不同给药时机对肿瘤侵袭转移的影响。肿瘤体积和增殖细胞核抗原阳性细胞率明显降低。对照组肿瘤呈4C级侵袭，而抗癌药物治疗组77.3%的小鼠肿瘤呈3级侵袭，并对肿瘤侵袭有抑制作用。术后第7天顺铂组和培普霉素组的颈淋巴转移率明显降低。转移淋巴结的肿瘤分期进展也受到抑制。化疗不仅能有效地缩小肿瘤，而且还能抑制肿瘤的侵袭和转移。考虑到这些影响，抗癌药物的使用可能在临床上非常有用。

Using an orthotopic Implantation model in which the invasion and metastasis of oral cancer can be reproduced, we investigated the inhibitory effects of anticancer agents on invasion and metastasis. A highly invasive and metastatic human oral squamous cell carcinoma cell line, OSC-19,was implanted into the oral floor of nude mice, and cisplatin or peplomycin was administered to the mice 7 or 14 days after the implantation. The effects of each anticancer drug and different administration timings on cancer invasion and metastasis were investigated. Tumor size and the ratio of proliferating cell nuclear antigen positive cells was significantly reduced. In the control group, the tumors showed grade 4C of mode of invasion, while in the groups treated with anticancer drugs, grade 3 was observed in 77.3% of the mice, with an inhibitory effect on tumor invasion being observed. The rate of metastasis in the cervical lymph node was significantly decreased in the groups treated with the cisplatin or peplomycin on day 7 after the implantation. The tumor stage progression in the metastatic lymph nodes was also inhibited. Chemotherapy is effective not only for tumor diminution, but also for inhibiting invasion and metastasis. The use of anticancer drugs considering these effects may be clinically very useful.