Development of artifical vascular system with bioabsorbable polymer

我们研究了我们使用高培养基摆线式培养装置进行细胞培养的载体对分培养细胞的供氧效率和气体饱和培养基的制泡作用。我们注意到作为载体的生物可吸收聚合物将培养的细胞移植到生物体和用于它们的生物材料，生物可降解外科缝合线和胶原线。对于这些材料，胶原线产生的载体的形状是多种多样的，与实验牙线相比，缺乏线的强度。我们使用培养装置进行的细胞培养检查了培养条件和胶原的影响。<methods>细胞载体实验产生它在一个简单的纹理成分使用可生物降解的外科缝合线，胶原线。细胞培养使用我们切除的骨髓进行孵育，我们测量了血细胞，并检查了培养基和胶原蛋白的影响。<results>考虑到不同的编织方式和材料的特性，实验性地生产了桶型和扁平型的细胞载体。采用细胞培养仪进行细胞培养，测定血细胞成分。至于红细胞和白细胞，在细胞培养物中的血细胞成分中发现了减少的趋势，而在大多数血红蛋白中则没有。此外，血小板接受增加，并显示与对照培养物相比增加。用分离的血液成分进行骨髓培养也显示出造血干细胞的增加趋势。血液水平等于或小于10%，并且随着时间的推移观察到细胞计数减少的趋势，但是20%-40%的水平显示造血干细胞增加的趋势。间质细胞的增加是不被接受的。

We investigated carrier of the cell culture which we used high culture medium cycloid type culture apparatus for of the oxygen supply efficiency that divided a culture cell and gas saturation culture medium making alveus. We paid attention to a bioabsorbable polymer as carrier to transplant cultured cells to organism and organism material to use for them, biodegradability surgical suture and a collagen thread. As for these material, the collagen thread produced carrier of the configuration which was various to lack intensity of a thread in comparison with floss experimentally. The cellular culture that we used culture apparatus for examined a culture condition and influence of collagen. <methods> Cell carrier produced it experimentally in a simple texture ingredient using biodegradable surgical suture, collagen thread. Cell culture incubated it using the bone marrow which we resected and we measured a blood cell and examined culture medium and influence of collagen. <results> Cell carrier produced a barrel and a flat type experimentally in consideration of various how to weave and characteristics of material. The cell culture was used with culture apparatus and the ingredient of blood cell was measured. As for the erythrocyte and the leucocyte, a tendency to decrease was recognized in a blood cell ingredient in cellular culture, and in most of the haemoglobin was not. In addition, blood platelet accepted increase and showed increase in comparison with control culture. And also the culture using discrete blood constituent for than bone marrow revealed an increase tendency to hematopoietic stem cell. A blood level was equal to or less than 10%, and a tendency to decrease of cell count was seen with a time course, but level of 20%-40% revealed an increase tendency to hematopoietic stem cell. And the increase of an interstitial cell was not accepted.