Development of the anti-oxidization activity measuring method using a few active oxygen species induced by microwave exposure and ultra weak bioluminescence

我们试图用微波照射诱导的几种活性氧来测量生物体的抗氧化活性，这些活性氧诱导的超弱生物发光（称为生物光子）。生物光子是由活性氧产生的微弱化学发光。活性氧具有剧毒，可被生物体的抗氧化系统（某些酶和抗氧化剂）灭活。因此，如果我们能够在生物体中产生活性氧，我们就可以通过生物光子测量来测量其抗氧化活性。一些研究人员报告说，微波暴露会诱发一些活性氧。为此，我们研制了用于生物光子测量的微波应用器，并证实了微波照射引起的生物光子发射增量。此外，我们还测量了电加热器引起的热冲击下的生物光子发射，以探讨微波暴露的主要热冲击效应。结果表明，温度升高越快，光子发射越强。因此，我们认为微波暴露的主要影响是局部加速加热。

We tried to measure the anti-oxidization activity of living organism using a few active oxygen species induced by microwave exposure that induce ultra weak bioluminescence (called biophoton). The biophoton is weak chemiluminescence originated from the active oxygen species. The active oxygen species are highly toxic, and inactivated by anti-oxidization system (some enzymes and antioxidants) of living organism. So if we can generate the active oxygen species in living organism, we can measure the anti-oxidization activity by the biophoton measurement.Some researchers report that microwave exposure induced some active oxygen species. Thus we made the microwave applicator for biophoton measurement, and confirmed the increment of biophoton emission induced by microwave exposure.Furthermore, we measured the biophoton emission under the heat shock occurred by an electric heater to investigate the heat shock that was the main effect of microwave exposure. As the results, faster temperature rise induced stronger biophoton emission. So, we concluded the main effect of microwave exposure was the localized quicker heating.