Combined estrogenic effect of soybean food containing phytoestrogens and endocrine disruptor

异黄酮，如染料木素和大豆苷元，是存在于大豆食品中的植物雌激素，具有雌激素活性。在本研究中，我们研究了含有人体摄入剂量水平异黄酮（10 mg/kg/天）的大豆提取物（Nijiru）作为大豆食品和内分泌干扰物的联合雌激素效应。将大豆在120℃的水中煮40分钟，制成二酒，然后冷冻干燥制成粉末。Nijiru粉末含有大豆苷元、糖苷元、染料木素、大豆苷元、糖苷元和染料木素。采用Nijiru （1900 ~ 6600 mg/kg，对应于总异黄酮10 mg/kg的剂量）连续11 d灌胃给药。合成内分泌活性激素己烯雌酚（DES） （0.05 mg/kg）与Nijiru联合口服，连续用药3 d。Nijiru+DES治疗的相对子宫重量（占体重的百分比）略高于单独DES治疗。同样，合成内分泌活性化合物双酚A (BPA) （300 mg/kg）或农药甲氧氯（MX） （50 mg/kg）与Nijiru共同口服，并持续服用3天。虽然Nijiru+MX治疗增加了相对子宫重量（与单独使用MX相比），但Nijiru+BPA治疗没有增加相对子宫重量（与单独使用BPA相比）。

Isoflavones, such as genistein and daidzein, are phytoestrogens present in soybean foods and have estrogenic activity. In this study, we examined the combined estrogenic effect of soybean extract (Nijiru) containing human intake dose level of isoflavones (10 mg/kg/day) as soybean food and endocrine disruptor.Soybeans were boiled for 40 min at 120"C in water, and Nijiru was produced and was then freeze-dried to make a powder. Nijiru powder contained daidzin, glycitin, genistin, daidzein, glycitein, and genistein. Nijiru (1900-6600 mg/kg corresponding to dose of total isoflavone 10 mg/kg) was administered to ovariectomized ICR mice by oral gavage for 11 consecutive days. The synthetic endocrine-active hormone diethylstilbestrol (DES) (0.05 mg/kg) was then co-administered orally with Nijiru and continued for another 3 days. The relative uterine weight (% of body weight) of Nijiru+DES treatment was slightly higher than that of DES alone. Similarly, synthetic endocrine-active compound bisphenol A (BPA) (300 mg/kg) or pesticide methoxychlor (MX) (50 mg/kg) were co-administered orally with Nijiru and continued for another 3 days. Although Nijiru+MX treatment increased (vs. MX alone) the relative uterine weight, Nijiru+BPA treatment did not increase (vs. BPA alone) the relative uterine weight.