Construction of self-assembled membranes that can support lipid bilayers

这个项目的主要目的是创造能够支持脂质双分子层的自组装膜（sam）。首先，研究者将环状脂质与低聚乙二醇偶联，合成了含巯基的脂质异构体。将金衬底（含金电极的石英晶体）浸在不同摩尔比的聚脂体和巯基醇的混合物中制备了SAMS。为了检验SAMs是否会固定水溶液中的脂质双分子层，研究者使用石英晶体微天平作为检测工具。即，将脂质体分散体置于附着在石英晶体上的金电极上的SAM上，并测量频率偏移。频率的降低表明SAM上的脂质双分子层固定。研究者检查了各种SAMS，发现由环状脂质聚脂体和巯基醇以2:98的比例组成的膜有效地固定了。

The primary aim of this project is to create self-assembled membranes (SAMs) that can support lipid bilayers. First, t investigator synthesized the lipooligomers containing thiol group by conjugating a cyclic lipid and oligoethyleneglycol. Th SAMS were prepared by dipping gold substrate (quartz crystal with gold electrode) into a mixture of the lipooligomer a thioalcohol with various molar ratios.To examine if the SAMs would immobilize lipid bilayers in aqueous solution, the investigator used quartz crystal microbalance as a detecting tool. Namely, liposome dispersion was placed on the SAM attached to gold electrode on a quartz crystal, and measured frequency veering. The decrease of frequency indicates immobilization of lipid bilayers on the SAM. The investigator examined various SAMS, found that the membranes that are composed of cyclic lipid lipooligomer and thioalcohol with a ratio of 2:98 effectively immobilized.