Development of separation processes using ionic liquids

1)在这项研究中，我们研究是否室温离子液体（RTILs）作为一种膜溶液可以用于从石油，有机酸和青霉素G从发酵液中分离烃类，通过使用支撑液膜。我们使用1-正丁基-、1-正己基-和1-正辛基-3-甲基咪唑的六氟磷酸盐和季铵盐作为离子液体。用离子液体浸渍聚偏氟乙烯多孔膜制备支撑液膜。在此基础上，成功地实现了有机硫、氮化合物、青霉素G和乳酸的跨膜传输。(2)对映体纯化学品在医药和农业中具有重要的应用，因为每种对映体对人体的活性不同。特别是手性二元醇是最优秀的中间体之一，因此需要对映体纯的制备方法。离子液体是一种新型的酶促反应介质，具有不挥发、易燃烧、热稳定、极性强等特点。在酶促反应中表现出很高的稳定性和对映选择性。本研究在缓冲液/离子液体两相体系中，利用具有对映选择性的环氧化物水解酶对外消旋环氧化物进行动力学拆分，制备对映体纯的二元醇。本研究使用了红酵母环氧化物水解酶，该酶对1，2-环氧己烷具有很高的对映选择性。红酵母ATCC 201718的环氧化物水解酶在不经全细胞提取的情况下表现出很高的催化活性，并且对R-对映体具有很好的对映体选择性。

1) In this study, we examine whether room-temperature ionic liquids (RTILs) as a membrane solution can be utilized for hydrocarbon separation from petroleum, and organic acid and penicillin G from the fermented broth by using supported liquid membrane. We used the hexafluorophosphates of 1-n-butyl-, 1-n-hexyl-and 1-n-octyl-3-methyl imidazolium and quaternary ammonium salts as an ionic liquid. The supported liquid membrane was prepared by impregnating the porous polyvinylidene fluoride film with the ionic liquid. Organic sulfur and nitrogen compounds, peniciliin G and lactic acid were successfully transported through the membrane based on the ionic liquids.(2) Enantiopure chemicals are important in pharmaceutical and agricultural, because each enantiomer shows different activity for human body. Specially, chiral diols are one of the most excellent compounds as intermediate materials, therefore enantiopure preparation method is required. Ionic liquid is a novel medium for enzymatic reactions and has characteristics such as nonvolatile, inflame ability, thermal stability and high polarity. It has shown high stability and enantioselectivity in enzymatic reaction. In this research, it was performed kinetic resolution of rac-epoxides by epoxide hydrolase, which has enantioselectivity, for preparing enantiopure diol in biphasic system of buffer/ionic liquid. Epoxide hydrolase from Rhodotorula glutinis, which had reported for high enantioselectivity for 1,2-epoxyhexane as substrate, was used for this research. Epoxide hydrolase from Rhodotorula glutinis ATCC201718 shows high catalytic activity without being extracted from whole-cell, and excellent enantioselectivity for R-enantiomer was confirmed.