Nano-particles which Contained Drug Induction into Tumor Cell by Electrochemotherapy and Sonoporation

1.抗癌药物纳米粒的制备除去年制备的多肽Poly [（GVGVP GVGVP GKGVP GVGVP GVGVP GVGVP）_（GVGVP）]外，我们还制备了多肽[（GVGVP）25]<22>，并在[（GVGVP）25]颗粒中浸渍了红色染料（Brebrich Scarlet Red）。两周多后，色素逐渐排出。我们让3。5mg甲氨蝶呤融合30mg肽。结果，MTX释放超过最小3天。2纳米颗粒结合抗癌药物和抗癌药物通过声孔进入肿瘤细胞的诱导和增强的抗肿瘤作用我们通过使用甲氨蝶呤组分的纳米颗粒在小鼠刘易斯肺癌中检测声孔的抗肿瘤作用的增强是否可行。将荷瘤小鼠分为五组：（1）无治疗（2）单独的甲氨蝶呤治疗（3）单独的甲氨蝶呤组分纳米颗粒治疗（4）甲氨蝶呤治疗然后声致孔（5）甲氨蝶呤组分纳米颗粒治疗然后声致孔。腹腔注射药物后，将声穿孔应用于肿瘤。治疗后每2天测量小鼠的肿瘤。肿瘤体积增大率4、5组与其他3组比较差异有显著性。特别是5号组，抗肿瘤作用增强。声孔组（4）、（5）肿瘤组织中甲氨蝶呤含量高于其他组。为了证实通过声孔作用促进甲氨蝶呤在体内肿瘤细胞中的诱导，将荧光缀合的MTX施用到肿瘤细胞中。在声致孔的存在下，观察到荧光阳性细胞，而在不存在声致孔的情况下仅观察到弱阳性区域。

1. Manufacture of nano-particles which contained an anti cancer drugWe made polypeptide [ (GVGVP) 25] besides the polypeptide Poly [ (GVGVP GVGVP GKGVP GVGVP GVGVP GVGVP)_<22>(GVGVP)] which made in last year and impregnated red dyes (Brebrich Scarlet Red) in the [ (GVGVP) 25] particle. Pigments were gradually discharged from them more than two weeks. We let 3. 5mg methotrexate fuse for the 30mg peptide. As a result, MTX was released more than minimum 3 days.2 Nano-particles conjugated anticancer drug and anticancer drug induction into tumor cell by sonoporation and enhanced antitumor effectsWe examined whether enhancement of the antitumor effect of sonoporation was feasible by using nano-particle of methotrexate component in mice Lewis lung carcinoma. The tumor bearing mice were divided into five groups : (1) no treatment (2) methotrexate treatment alone (3) nano-particle of methotrexate component treatment alone (4) methotrexate treatment followed by sonoporation(5)nano-particle of methotrexate component treatment followed by sonoporation. Sonoporation was applied to the tumors after intraperitoneal injection of drugs. Tumors of the mice were measured every 2 days after treatment. Differences in the rate of tumor volume increase between No. (4), (5) groups and other three groups were significant. As for No. (5) group in particularly, antitumor effect was enhanced. The methotrexate content of the tumor tissues from the sonoporation groups (No. (4), (5)) were higher than other groups. To confirm the promotion of methotrexate induction in vivo tumor cells by sonoporation, fluorescence conjugated MTX was administered into tumor cell. In the presence of sonoporation, fluorescence-positive cells were observed, while only a weakly positive area was observed in the absence of sonoporation.