Gene expression assay using Microarray technique between embryonic stem cell and inner ear sphere formation cells.

在人的内耳中，在Corti和螺旋神经节细胞器官中毛细胞丢失后，毛细胞和神经节细胞不能自发再生。以前，已经证明细胞移植到受损的内耳是永久性听力损失的候选治疗方法。但是，在细胞移植的细胞来源保障方面存在着严重的问题。据报道，胚胎干细胞具有向毛细胞表型分化的潜力。胚胎干细胞是克服这些问题的可能的候选者。为了开发诱导胚胎干细胞向内耳毛细胞和螺旋神经节神经元分化的方法，我们需要了解胚胎干细胞与内耳毛细胞和神经节神经元的区别。在这项研究中，我们比较了胚胎干细胞和从发育中的小鼠内耳获得的细胞培养中表达的基因。首先，我们建立了含有未成熟内耳细胞的胚胎10天小鼠内耳球形细胞培养物。其次，利用Agilent阵列分析胚胎干细胞和胚胎球形细胞培养物的表达基因，并进行环蒂分析。结果表明，与胚胎干细胞培养相比，与神经谱系相关的基因，如Olig1和01ig2，在胚胎干细胞培养中表达得更多。这项研究可能会引导我们开发新的方法，从胚胎干细胞中产生毛细胞和神经节神经元。在此期间，我们致力于听力损失动物模型和细胞移植的研究（Imura等）。《印刷科学2007》，Sekiya等人。实验神经病学[J] .中华神经病学杂志[J] . 25(2): 23 -24, 2005。

In the human inner ear, after loss of hair cell in the organ of Corti and spiral ganglion cells, the hair cells and ganglion cells do not regenerate spontaneously. Previously, it has been shown that cell transplantion to the damaged inner ear is a candidate therapy for permanent hearing loss. But, we have severe problems on securing of cell source for the cell transplantation. It was reported that embryonic stem cells possess potential to differentiate into hair cell phenotypes. The embryonic stem cells are possible candidate to overcome the problems. To develop methods of induction of differentiation of embryonic stem cell into the inner ear hair cells and spiral ganglion neurons, we need to know differences between the embryonic stem cells and the hair cells and ganglion neurons. In this study, we compared genes expressed in embryonic stem cells and cell cultures obtained from mouse developing inner ear. First, we established sphere formation cell cultures from embryonic day 10 mouse inner ears which containing immature inner ear cells. Second, expression genes of the embryonic stem cells and the otic sphere forming cell cultures were profiled using Agilent array, and then analyzed cyclopedically. The results of this assay showed that genes related in neural lineages, e. g. Olig1 and 01ig2, were expressed in otic shere forming cell cultures more predominantly than embryonic stem cell cultures. The profiles made in this study may lead us to develop new procedures to generate hair cells and ganglion neurons from embryonic stem cells. In this period, we contribute to researches of animal models of hearing loss and cell trasnplantation (Imura et al. Scinece 2007 in printing, Sekiya et al. Experimental Neurology 198: 12-24, 2005, Sekiya et. al. Eur J Neurol 25 : 2307-18, 2007).