The effect of inflammatory cytokine for gastrointestinal motility

1.目的：观察炎性细胞因子(IL-1、β、IL-6)对豚鼠结肠带绦虫和先天性巨结肠模型大鼠结肠运动的影响。材料与方法：将残存的纵肌组织标本切成1×5×0.5 mm的条状。以乙酰氧甲酯(Fura-2/AM)的形式将钙离子指示染料Fura-2以乙酰氧甲酯(Fura-2/AM)形式负载豚鼠结肠带。用前表面荧光仪CAM-OF3(日本光谱仪)同时监测与Fura-2负载的结肠带结合的Ca~(2+)和~(2+)的荧光强度的变化。CO，东京，日本)。结果：IL-1、β和IL-6对卡巴胆碱引起的豚鼠结肠带收缩无明显作用。未见细胞内钙离子浓度升高。这些结果是由于IL-16和IL-6引起的血管内皮细胞紊乱所致。试条在IL-16和IL-6中孵育的时间可能过长。另一方面，我们对先天性巨结肠模型大鼠肠平滑肌的运动功能进行了研究。在无神经节细胞的肠平滑肌中，P物质引起的收缩幅度的升高低于正常的肠平滑肌。这一结果被认为是由于无神经节细胞膜上钙通道的抑制所致。为了研究Rho激酶在无神经节细胞中的作用，我们使用了Rho激酶抑制剂(Y-27632)。在无神经节细胞的肠道中，Rho激酶途径是正常的。目前尚不认为Rho激酶通路是先天性巨结肠肠道功能紊乱的原因。

1. Aim : To investigate the effect of inflammatory cytokine (IL-1β, IL-6) for intestinal motility, we simultaneously measured intracellular calcium concentration and tension in guinea pig taenia coli and Hirschsprung disease model rat colon.2. Materials ＆ methods : The residual longitudinal muscle tissue preparation was cut into strips (1×5×0.5 mm). the guinea-pig taenia coli strips were loaded with Ca^<2+> indicator dye, fura-2 in the form of acetoxymethyl ester (Fura-2/AM). The changes in the fluorescence intensity of Ca^<2+> binding with fura-2 loaded taenia coli strips were simultaneously monitored with a front-surface fluoromater CAM-OF3 (Japan Spectroscope. Co, Tokyo, Japan).3. Results : IL-1β and IL-6 are not effective for the contraction induced by calbachol in guinea pig taenia coli. The intracellular calcium concentration elevation was not observed. These result was due to the disorder of the smooth muscle cells with IL-16 and IL-6. The strips might be incubated in IL-16 and IL-6 too much time. On the other hand, We studied about the motility of the Hirschsprung disease model rat intestinal smooth muscle. In the aganglia intestinal smooth muscle, the elevation of contraction induced by substance P was lower than in the normal intestinal smooth muscle. This result is assumed to be due to the depression of the calcium channel on the aganglia smooth muscle cell membrane. To investigate the Rho kinase pathway in aganglia intestinal smooth muscle, we used Rho kinase inhibitor (Y-27632). In aganglia intestine, Rho kinase pathway is normal. It is not assumed that Rho kinase pathway is the cause of intestinal disorder in Hirschsprung disease.