Arginine effect on the gastrointestinal motility in the growing rat after massive small bowel resection

【目的】采用适合研究精氨酸在体内重要性的短小肠模型，不加限制地记录胃、十二指肠、空肠和回肠的运动功能，并与对照组进行比较。【对象与方法】1)4周龄雄性大鼠，行90%近端小肠切除术（SBR），对照组切肠，在回盲瓣口5cm处吻合。对接受手术的大鼠和小肠长度正常的大鼠分别给予含有精氨酸或甘氨酸的Elental-P。按此方法共制备四组。2)术后第三周，切开腹部，将力传感器（FT）缝合于胃幽门部分浆膜处，十二指肠距幽门环3 cm处，空肠距Treitz韧带3 cm处，回肠距吻合口2 cm处。从三天后开始，用多通道遥测系统（Star医疗公司）记录胃肠运动。3)禁食12 ~ 24小时后，反复记录并分析1 ~ 3天胃肠运动。单次试验周期为12小时喂养和12小时禁食。实验结束后处死大鼠，确保FTs放置正确。[结果]1)对照组摄食前十二指肠每隔3-5分钟出现持续3-5分钟的迁移运动复合物（MMC），空肠每隔3-4分钟出现持续3-4分钟的迁移运动复合物（MMC）。开始饲喂后，MMC消失。然而，MMC在3-4小时后出现，并以低频持续。MMC在开始饲喂12 h后逐渐增加，18 h后频率变为每3-5 min出现一次。每次给药都重复相同的MMC模式。2) SBR组在饲喂前十二指肠出现持续3 ~ 5 min的MMC，但在开始饲喂后消失。3 ~ 4小时后，每1 ~ 2小时再次观察到MMC， 18小时后每8 ~ 10分钟观察到MMC持续2 ~ 3分钟。发生在十二指肠的MMC不能很好地转移到其余的回肠。3)对照组和SBR组均未见MMC通过吻合口向回肠明显转移。[结论]根据目前的观察，SBR后MMC在残余回肠中的清洁作用似乎被抑制。在我们的模型中，精氨酸对胃肠道运动没有影响。少

[Objective] Using our short-small intestine model that is suitable for investigating the importance of arginine in vivo, the motor functions of the stomach, duodenum, jejunum, and ileum were recorded without any restraint, and the results were compared to those of control rats.[Subjects and Methods] 1) Using 4-week-o1d male rat, 90% proximal small bowel resection (SBR) was performed, and in the control group, the intestine was cut and then anastomosed at 5 cm oral to the ileocecal valve. Either Elental-P with arginine or glycine was administered to rats that underwent this surgery and rats with a normal length of smal1 intestine. A total of four groups were prepared in this manner. 2) On the third week after surgery, the abdomen was reopened and a force transducer (FT) was sutured to the serous membrane of the pyloric portion of the stomach, the duodenum 3 cm anal to the pyloric ring, the jejunum 3 cm ana1 from Treitz ligament, and the ileum 2 cm anal to the anastomosis. From three day … More s later, gastrointestinal motility was recorded using a multichannel telemeter system (Star Medical Co.). 3) After fasting for 12 to 24 hours, gastrointestinal motility was repeatedly recorded and analyzed for 1-3 days. A single test cycle consisted of 12 hours of feeding and 12 hours of fasting. After the end of the test, the rats were sacrificed to ensure that the FTs were positioned properly.[Results] 1) For the control groups, immediately before feeding, migrating motility complexes(MMC) lasting 3-5 minutes were seen every 3-5 minutes in the duodenum, and then MMC lasting 3-4 minutes were seen in the jejunum. MMC disappeared with the start of feeding. However, MMC appeared 3-4 hours later and persisted at low-frequency. MMC increased gradually from 12 hours after the start of feeding and their frequency changed to every 3-5 minutes after 18 hours. The same MMC patterns were repeated with every administration. 2) For the SBR groups, immediately before feeding, MMC lasting 3-5 minutes were seen in the duodenum, but these disappeared with the start of feeding. Three to four hours later, MMC were again seen every 1-2 hours and MMC lasting 2-3 minutes were observed every 8-10 minutes from 18 hours later.MMC that occurred at the duodenum did not transmit well to the remaining ileum. 3) For both control and SBR groups, clear transmission of MMC to the ileum over the anastomosis was not seen.[Conclusion] Based on the current observations the housekeeping role of MMC in the residua1 ileum appears to be suppressed after SBR. There was no arginine effect on the gastrointestina1 motility in our model. Less