Understanding how animals generate and regenerate cell diversityintegrating live imaging, lineage tracking, deep learning and big image visualization

多细胞生物体是由各种各样的细胞组成的，所有这些细胞都是在发育过程中由单个细胞产生的。这些细胞是在细胞谱系(谱系)的背景下产生的，这在决定它们的最终命运和可塑性方面发挥着重要作用。一些动物还可以在一生中从身份/特性基本上未知的祖细胞中再生自己的身体。我们的目标是为确定和分析细胞谱系提供一个普遍的框架，揭示细胞谱系如何影响胚胎和再生成人的细胞命运。在实验方面，我们将开发遗传和活体成像方法来记录细胞谱系，重点是甲壳类动物夏威夷甲壳动物，这是一种实验模型，再生过程非常逼真。在计算方面，我们将开发一个用于细胞跟踪(包括深度学习)、可视化渲染和细胞谱系分析的集成框架。

Multicellular organisms are made of a wide diversity of cells, all generated from a single cell during development. These cells are generated in the context of a cell genealogy (lineage), which plays an important role in determining their ultimate fate and plasticity. Some animals can also regenerate their body throughout their lifetime, from progenitor cells whose identity/properties are largely unknown. Our goal is to provide a generalised framework for determining and analysing cell lineages, to reveal how cell genealogy impacts cell fate in embryos and regenerating adults. On the experimental side, we will develop genetic and live imaging approaches to record cell lineages, focusing on the crustacean Parhyale hawaiensis, an experimental model where regeneration occurs with exceptional fidelity. On the computational side, we will develop an integrated framework for cell tracking (including deep learning), visual rendering and analysis of cell lineages.