Systematic Profiling of Genetic Components of Inflammasome Activation during Metaflammation (P21#)

肥胖引起的慢性炎症是非传染性疾病的主要风险因素。虽然碳水化合物和脂肪过量供应引起慢性炎症的机制尚不完全清楚，但许多非传染性疾病的小鼠模型指出了单一炎症关键途径-NLRP3炎症小体的共同作用。该项目旨在利用基于CRISPR的功能基因组学技术，系统地描述导致人类细胞NLRP3激活的所有分子事件，以响应代谢条件、细胞危险信号和病原体感染。

Obesity-induced chronic inflammation is a major risk factor for non-communicable diseases. While the mechanism of chronic inflammation caused by over-supply of carbohydrates and fat is not fully understood, mouse models of numerous non-communicable diseases point to a common role of a single inflammatory key pathway, the NLRP3 inflammasome. This project aims to systematically delineate all molecular events leading to NLRP3 activation in human cells in response to metabolic conditions, cellular danger signals, and pathogen infections, using CRISPR-based functional genomics technologies.