Bio-organic Chemical Studies on the Floral Scent Formation and Flower Induction

1.月季花的昼夜香味释放。在四季蔷薇（Rosa quatra saison）和四季蔷薇（R. 1.2.葡萄糖苷酶抑制剂在2-苯基乙醇释放中的作用在开始喂食β-葡萄糖苷酶抑制剂后的白天，2-苯基乙基β-D-吡喃葡萄糖苷（2）的水平在喂食组中保持相当稳定，但在对照组中在喂食开始时下降，但在进食组中，β-葡萄糖苷酶的浓度在展开过程中几乎恒定。从玫瑰花瓣中获得了一个β-葡萄糖苷酶克隆，但其功能尚未阐明。3. 1和2 [^2H_8]-1和[^]的生物发生[^2H_8]-2必须由[^2H_8] L-苯丙氨酸生物合成，氘原子保留在[^2H_8] L-苯丙氨酸的α-位上。4.克隆与花香化合物生物合成有关的O-甲基转移酶。从玫瑰花瓣中分离纯化了一个新的类胡萝卜素降解酶（OMT），并利用MALDI-TOFMS对其部分序列进行了分析，证实了玫瑰花中存在类胡萝卜素降解酶。5. KODA，一种与牵牛花诱导有关的化合物。（与横山博士合作研究，资生堂株式会社）首次从牵牛属植物诱导成花的幼苗中鉴定出α-酮醇亚麻酸（KODA通过LC-SIM和LC-MS/MS分析，对KODA的一种代谢产物进行了LC-MS-MS和NMR表征。

1.Diurnal Floral Scent Emission form flowrs of Rosa damascena, R.'Hoh-Jun' Rhythmic cycles in the emission of 2-phenyletanol (1) are observed in Rosa damascena quatra saison and R.'Hoh-Jun', although R.damascena Mill.transiently emits 1.2.Role of glucosidase inhibitor in 2-phenylethanol emission During the day-time after the feeding of β-glucosidase-inhibitor commenced, the level of 2-phenylethyl β-D-glucopyranoside (2) remained fairly constant in the fed group, but decreased in feeding commenced in the control group, but the concentration was almost constant during the unfurling process in the fed group One β-glucosidase clone was obtained from the petals of Rose damascena Mill.although its function is not clarified yet.3.Biogenesis of 1 and 2 [^2H_8]-1 and [^2H_8]-2 must be biosynthesized from [^2H_8] L-phenylalanine with a retention of the deuterium atom at α-position of [^2H_8] L-phenylalanine.We fed deuterium-labeled intermediates into R.'Hoh-Jun' flowers.A high incorporation of phenylacetaldoxime was confirmed.We have proposed a plausible biosynthetic pathway for 1 and 2.4.Cloning of O-methyltransferases involved in the biosynthesis of floral scent compounds. New OMT, POMT, was purified from flower petals, and characterized the partial sequences by MALDI-TOFMS.We also confirmed the presence of carotenoid cleavage enzymes in rose flowers.5.KODA, a compound involved in flower-induction of Pharbitis nil.Violet (collaborative reseearch with Dr.Yokoyama, Shiseido Co. Ltd) α-Ketol linolenic acid (KODA) was identified for the first time in seedlings of Pharbitis grown under a flower-inductive condition (16-h dark exposure), by means of LC-SIM and LC-MS/MS analyses.One of the metabolites of KODA has been characterized by LC-MS-MS and NMR.