A novel detection system based on amplification and a strongly correlated hydrogen network

从聚合物化学的观点来看，DNA被认为是一种强相关的软材料，因为它的结构是由许多协同作用决定的，如氢键、芳环的pi-pi堆积和静电力。利用DNA的这些特性，可以成功地开发出一种新材料。或者，可以利用DNA本身来开发DNA合成技术。到目前为止，我们站在后者的立场上，一直在研究含有生化有用试剂的材料。在这里，我们讨论了一种新的试剂，它利用DNA的四链形成，这是DNA复杂结构的一种形式。1)钾敏感寡核苷酸、粒子群算法几种金属离子在生物体中具有重要作用。钾离子与其他金属离子及某些疾病的调节有关，在医学领域备受关注。到目前为止，已有几种试剂在这一点上进行了研究，但它们的水溶性和特异性都不够。在本论文中，我们首次研制成功了一种钾离子的水溶敏感试剂，该试剂对钾离子比钠离子具有很高的特异性。2)TAP作为一种潜在的端粒酶抑制物某些癌症可能是由于端粒酶活性增强所致，端粒酶催化端粒DNA末端的延伸。由于端粒酶不能与G-四元组结构结合，因此能够稳定这种DNA结构的化合物是很好的端粒酶抑制剂。我们检测了与DNA相互作用的TAP对端粒酶的抑制能力。TAP具有显着的G-四联体结构的稳定能力，因此有望成为一种新型的端粒酶抑制剂。

DNA is regarded as a strongly correlated soft material from a viewpoint of polymer chemistry, as its structure is dictated by many cooperative interactions such as hydrogen bonding, pi-pi stacking of aromatic rings, and electrostatic forces. A new material may be developed successfully using these characteristics of DNA. Alternatively, DNA itself may be utilized aiming at developing a DNA synthesizing technology. So far, we stand in the latter position and have been studying materials containing biochemically useful reagents. Here, we discuss a new reagent by using DNA tetraplex formationwhich is one form of complex structures of DNA. 1)Potassium sensing oligonucleotide, PSO Several metal ions have an important role in living organism. Potassium ion is connected with regulation of other metal ions and some diseases and is attracted in the medical area. So far several reagents were studied in this point, but they have insufficient water-solubility and specificity. In this paper, we firstly succeeded the water-soluble sensing reagent for a potassium ion, which showed the very high specificity for potassium ion over sodium one. 2)Tetrakis-acridiny peptide (TAP) as a potential telomerase inhibitor Some of the cancers are believed to be derived from enhanced activity of telomerase, which catalyzes the extension of telomere DNA termini. Since telomerase cannot bind to a G-quartet structure, compounds which can stabilize such a DNA structure are good candidate for telomerase inhibitor. We tested inhibition ability of TAP, which interacts with DNA, for telomerase. TAP had remarkable stabilization power of a G-quartet structure and hence is promising as a novel telomerase inhibitor.