Characterization of roles of PRL-CDC5 complex in regulation of pre-mRNA splicing and flowering time

CDC 5 Myb因子与PRL型WD-40蛋白的相互作用是真核生物中剪接体组装和激活所必需的。我们的数据表明，prll和cdc 5突变引起类似的多个发育和信号通路的调节，包括开花时间的控制的同时变化。拟议的研究旨在表征前体mRNA剪接缺陷，转录控制和由prll和cdc 5突变引起的开花时间和花发育调节的改变之间的因果关系，以及鉴定PRL/CDC 5交联的前体mRNA和CDC 5 Myb结合的染色质靶标。

Interaction of CDC5 Myb factors with PRL-type WD-40 proteins is required for Spliceosome assembly and activation in eukaryotes. Our data show that prll and cdc5 mutations cause similar simultaneous changes in the regulation of multiple developmental and signaling pathways including the control of flowering time. The proposed studies aim at the characterization of causal relationships between defects in pre-mRNA splicing, transcription control and alterations in the regulation of flowering time and flower development caused by the prll and cdc5 mutations, as well as the identification of PRL/CDC5 cross-linked pre-mRNA and CDC5 Myb-bound chromatin targets.