Mass spectrometer with MALDI2 and ESI ion source,Laser Capture Microdissection device

成像方式有可能破译免疫细胞的分布和组织中的局部微环境。成像数据集的注册可能是未来全面揭示炎症过程中细胞和组织特定机制的关键。在IMCES，最先进的显微镜已经被用来确定免疫细胞的分布。通过在这种大规模设备应用的背景下建立局部质谱学，不仅可以确定免疫细胞在组织中的分布，而且可以将这些分布模式注册到局部蛋白质组中。因此，我们将对疾病中介导白细胞渗透和炎症状态的局部分子机制提供新的见解。挑选在免疫学、传染病、神经学、癌症和内分泌学方面具有互补专业知识的项目负责人，将有助于解开各种疾病的疾病、器官和细胞特有的机制。相应的研究主题目前正在由现有和拟议的DFG联合体的项目负责人处理，并代表了杜伊斯堡-埃森大学医学院目前的研究重点。埃森通过本地质谱学的成像能力的扩展代表了IMCES对最先进成像方式的持续进一步发展。

Imaging modalities have the potential to decipher the distribution of immune cells and the local microenvironment in tissues. The registration of imaging datasets could be the key to comprehensively unravelling the cell and tissue specific mechanisms in inflammatory processes in the future. At IMCES, state-of-the-art microscopes are already being used to determine the distribution of immune cells. By establishing local mass spectrometry in the context of this large-scale equipment application, it will be possible not only to determine the distribution of immune cells in tissues, but also to register these distribution patterns with the local proteome. Thus, novel insights of the local molecular mechanisms that mediate leukocyte infiltration and inflammatory conditions in diseases will be provided. The selection of project leaders with complementary expertise in immunology, infectious diseases, neurology, cancer and endocrinology will allow unraveling disease-, organ-, and cell-specific mechanisms in various diseases. The corresponding research topics are currently being addressed by the project leaders in existing and proposed DFG consortia and represent the current research focus of the Medical Faculty of the University of Duisburg-Essen. The expansion of the imaging capabilities in Essen via local mass spectrometry represents a consistent further development of state-of-the-art imaging modalities at IMCES.