The role of late endolysosomal transport in podocyte functions.

内吞和自噬之间的失衡在肾脏疾病的发病机制中起着重要作用。我们的假设是，晚期内溶酶体运输/降解途径对足细胞具有特定的功能。为了验证这一假设，我们在体内敲除了小鼠足细胞中的GTPase Rab7，这是晚期内溶酶体和自溶酶体运输的关键酶。为了验证这一假设，我们在体内敲除了小鼠足细胞中的GTPase Rab7，这是晚期内溶酶体和自溶酶体运输的关键酶。具有足细胞特异性Rab7基因敲除的小鼠发生足细胞损伤，并成为蛋白尿。利用细胞生物学技术，我们旨在了解这种足细胞损伤的机制；特别是我们旨在研究足细胞的功能和信号通路，以及它们在Rab7基因被敲除后的内溶酶体转运，包括溶酶体功能。该项目将对晚期内溶酶体运输对足细胞功能的作用提供重要的见解。

Disturbed balance between endo- and autophagocytosis plays an important role in the pathogenesis of renal diseases. Our hypothesis is that the late endolysosomal transport/degradation pathway has specific functions for podocytes. To test this hypothesis, we knocked down the GTPase Rab7, a key enzyme of late endolysosomal and autolysosomal transport, in mouse podocytes in vivo. To test this hypothesis, we knocked down the GTPase Rab7, a key enzyme of late endolysosomal and autolysosomal transport, in mouse podocytes in vivo. Mice with a podocyte-specific knockout of Rab7 developed podocyte injury and became proteinuric. Using cell biology techniques, we aim to understand the mechanisms of this podocyte injury; in particular, we aim to investigate podocyte functions and signaling pathways, their endolysosomal transport including lysosome function after knockdown of Rab7. The project will provide important insights into the function of late endolysosomal transport for podocyte function.