Biomedical Application of Gas Biology through Multidisciplinary Approaches

气体行为和气体目标的多样性，以及测量当地气体浓度的困难，掩盖了气体发挥其作用的详细机制，许多问题仍然没有得到回答。在这个项目中，先进的质谱学与高性能计算机技术相结合，使我们能够挖掘气体响应信号转换系统的生物机制。我们还通过考虑CO、NO和H_2S对代谢系统和能量支持酶系统(包括线粒体呼吸的关键靶标和中心介体细胞色素C氧化酶)的生理意义，对气体的相互作用采取了综合的方法。通过从体外和体内两个角度评估气体介导的控制功能，我们试图阐明O(2)、NO、CO和H2S的复杂的多重相互作用

The multiplicity of gas actions and gas targets and the difficulty in measuring local gas concentrations obscures detailed mechanisms whereby gases exert their actions, and many questions remain unanswered. In this project, advanced mass spectrometry combined with high-performance computer technology allowed us to mine biological mechanisms for gas-responsive signal transducing systems. We also take an integrated approach to the interaction of gases by considering the physiological significance of CO, NO, and H2S on metabolic systems and energy-supporting enzyme systems including cytochrome c oxidase, a key target and central mediator of mitochondrial respiration. By evaluating gas-mediated control functions from both in vitro and in vivo perspectives, we attempted to elaborate on the complex multiple interactions of O(2), NO, CO, and H2S