Comparing osmotic stress responses between wild barley cultivar, landrace and domesticated barley: chemical, physiological and molecular adaptations of apoplastic barriers in leaves and roots

在这个项目中，我们将比较野生大麦、地方大麦和驯化大麦，研究叶和根中的质外体屏障(表皮角质和蜡质沉积)和根质外体屏障(内皮层Suberin沉积)对渗透胁迫的适应性。这一方法包括(I)大麦叶片和根的质外体障碍的显微和化学分析研究，(Ii)大麦叶片和根长度的基因表达(转录)研究，(Iii)大麦叶片和根的代谢和离子研究，以比较质外体细胞壁反应和共体细胞反应，最后(Iv)大麦叶片和根质外体障碍的水分运输的定量分析。通过比较野生大麦、地方品种和驯化大麦，可以更深入地了解大麦叶片和根质外体屏障对渗透胁迫的适应。这些知识可能有助于未来大麦的育种，以获得更耐逆境的品种。

In this project we will investigate the adaptations of apoplastic barriers in leaves (epidermal cutin and wax deposition) and roots (endodermal suberin deposition) to osmotic stress comparing wild barley, landraces and domesticated barley. This approach includes (i) the microscopic and chemical-analytical investigation of apoplastic barriers over the length of barley leaves and roots, (ii) the investigation of the gene expression (transcriptomics) over the length of barley leaves and roots, (iii) the metabolomic and ionomic investigation of barley leaves and roots in order to compare apoplastic cell wall responses with symplastic cellular responses and finally (iv) the quantitative analysis of water transport across barley leaf and root apoplastic barriers. By comparing wild barley, landraces and domesticated barley, deeper insights in the adaptation of barley leaf and root apoplastic barriers to osmotic stress can be obtained. This knowledge could help in future in barley breeding to achieve more stress tolerant cultivars.