Structur and function of mutants of the 20S proteasome from S. cerevisiae (Struktur und Funktion von Mutanten des 20S Proteasoms aus S. cerevisiae)

基于1997年确定的酵母20S蛋白酶体的晶体结构，我们想要研究突变体和多肽复合体的结构和功能，特别是那些影响底物进入蛋白水解室的结构和功能。我们希望通过观察分离的组分或蛋白质复合体的结构来确定蛋白酶体相关蛋白的作用。特别是，我们想要集中在酵母和古细菌蛋白酶体的ATPase亚基上。还将对真细菌蛋白酶体类似物Hs1VU进行结构分析。

Based on the crystal structure of the 20S proteasome from yeast, determined in 1997, we want to investigate structure and function of mutants and peptide complexes, with special emphasis on those, that influence substrate access to the proteolytic chamber. We would like to determine the role of proteasome associated proteins looking at the structure of either isolated components or protein complexes. In particular, we want to focus on the ATPase subunits of the yeast and archaebacterial proteasomes. The eubacterial proteasome analogue Hs1VU will also be analysed structurally.