Influence of distraction rate on regenerate formation during femoral callus distraction in the rat model

节段性骨缺损是骨科和创伤学中最大的挑战之一，骨运输是最有效的治疗方法。尽管早已建立了骨痂牵张术，尽管新的种植体不断发展，但治疗的持续时间可能是几个月到几年，因为最佳的牵张率约为每天1毫米。因此，本动物实验的目的是质疑在分心阶段开始和结束时的最佳分心率。实验计划选用180只雄性布朗-挪威大鼠（来自德国Sulzfeld Charles River），随机分为3组。所有动物的分散距离为9毫米。各组的不同是基于传输速度（恒定vs先快后慢vs先慢后快）。分子（qRT-PCR）、放射学（μ- ct扫描）、生化（ELISA’s）和组织学（丙烯酸酯-组织学）检测将对再生质量进行研究。对于不同的分析，定义了四个时间点。

Segmental bone defects represent one of the greatest challenges in orthopedics and traumatology, with bone transport being the most effective treatment method. Although callus distraction has long been established and despite the constant development of new implants, the duration of treatment can be months to years, since an optimal distraction rate of approximately 1 mm per day has been taken for grated. Therefore, the aim of this animal experiment is to question the optimal distraction rate at the beginning and at the end of the distraction phase. The experiments are planned with 180 male Brown-Norway rats (Charles River, Sulzfeld, Germany), which will be randomly divided into 3 groups. Distraction distance is 9mm for all animals. The groups differ on the basis of the transport speed (constant vs. first fast, then slow vs. first slow, then fast. Molecular (qRT-PCR), radiological (μ-CT-Scans), biochemical (ELISA’s) and histological (Acrylat-histology) tests are planned to investigate the regenerate quality. For the different analyses four time points have been defined.