Sacrificial templated grafts to encourage bone healing through mechanotransduction

牺牲模板移植物通过机械传导促进骨愈合

• 批准号: 10811305
• 负责人: JOO L. ONG
• 金额: $ 41.17万
• 依托单位: UNIVERSITY OF TEXAS SAN ANTONIO
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-18 至 2025-09-17
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10811305
• 关键词: 3D Print; Affect; Anatomy; Autologous; Biocompatible Materials; Biological; Biomechanics; Bioreactors; Blood Vessels; Bolus Infusion; Bone Regeneration; Bone Tissue; Bone Transplantation; Bone callus; Bone plates; Breathing; Calcium; Cancerous; Cell Density; Cells; Chemotactic Factors; Clinical; Collagen; Congenital Abnormality; Coupled; Coupling; Data; Defect; Development; Disease; Distraction Osteogenesis; Engineering; Ensure; Environment; Esthetics; Excision; Exhibits; Face; Film; Goals; Growth Factor; Head; Health Care Costs; Hematopoietic stem cells; Implantation procedure; In Situ; In Vitro; Individual; Injury; Intervention; Jaw; Liquid substance; Maintenance; Mandible; Mastication; Mechanical Stimulation; Mechanics; Mesenchymal Stem Cells; Modeling; Morbidity - disease rate; Natural regeneration; Neck; Operative Surgical Procedures; Osteogenesis; Pain; Pathway interactions; Patients; Perfusion; Periodicity; Physiological; Polymers; Porosity; Printing; Process; Psychological Stress; Quality of life; Rattus; Research; Savings; Shapes; Signal Transduction; Site; Skeleton; Speech; Surface; Surgeon; System; Techniques; Thick; Time; Tooth Extraction; Traumatic injury; Vascularization; Work; angiogenesis; bone; bone fracture repair; bone healing; bone loss; calcium phosphate; cancer therapy; cell motility; craniofacial; craniofacial bone; craniomaxillofacial; design; efficacy study; functional restoration; healing; improved; in vivo; innovation; instrumentation; mechanical load; mechanical properties; mechanotransduction; meetings; novel; osteogenic; particle; public health relevance; recruit; regenerative; response; restoration; sample fixation; scaffold; substantia spongiosa

Sacrificial Templated Grafts to Encourage Bone Healing Through Mechanotransduction Project Summary Loss of large volumes of bone tissue often occurs as a result of traumatic injuries or cancerous resection. Such injuries, especially in the head, jaws, and neck are challenging to surgeons and the long- and short-term complications as well as the quality of life for these patients are often affected due to alteration in facial aesthetics and features, speech, mastication, and breathing. To overcome these complications, current clinical alternatives include grafting of bone with associated vascular bundles obtained from a secondary site. All these treatments require multiple clinical interventions and often result in increased pain or donor site morbidity for the patients. In this study, single-surgical step synthetic graft alternatives with controlled mechano-modulation through compliance changes within the scaffold as well as a concerted delivery of cell migration factors to enhance bone regeneration will be developed. As such, the 2 aims for this project are 1) to develop a chemoattractant incorporated bone graft substitute with engineered degradation zones while retaining its mechanical integrity for functional stabilization, and 2) to assess controlled temporal degradation under physiologically relevant conditions in vitro and mechanisms of osteogenic and angiogenic signaling through mechanotransduction and Ca2+ coupled pathways. Data collected from the in vitro and in vivo studies will guide the development of this novel graft and provide an established platform to study the effects of local micromechanical factors and release of recruitment factors to promote fracture healing and bone regeneration without need for extensive bone fixation instrumentation.

通过机械传导促进骨愈合的牺牲模板移植物 项目摘要 大量骨组织的丢失通常是由于创伤性损伤或癌性切除而发生的。 这种损伤，尤其是头部、颌部和颈部的损伤，对外科医生和长期和短期的治疗都是具有挑战性的。 这些患者的并发症以及生活质量常常由于面部美学的改变而受到影响 和特征，语言，咀嚼和呼吸。为了克服这些并发症，目前的临床替代方案 包括移植具有从第二部位获得相关维管束的骨。所有这些处理 需要多种临床干预，并且常常导致患者疼痛增加或供体部位发病率增加。 在这项研究中，单手术步骤合成移植替代品与控制机械调制，通过 支架内的顺应性变化以及细胞迁移因子的协同递送，以增强骨 再生将得到发展。因此，本项目的两个目标是：1）开发一种化学引诱剂 将骨移植替代物与工程降解区结合，同时保持其机械完整性， 功能稳定，以及2）在生理相关条件下评估受控的时间降解 体外条件和通过机械转导的成骨和血管生成信号传导的机制， Ca2+耦合途径。从体外和体内研究中收集的数据将指导本发明的开发。 新的移植物，并提供了一个既定的平台，以研究局部微机械因素和释放的影响， 募集因子，促进骨折愈合和骨再生，无需广泛的骨固定 仪器仪表