Time-resolved fluorescence and surface-enhanced Raman scattering spectroscopy microscope

对细胞内分析物(主要位于内质体/溶酶体)的传感将通过内吞作用、微量注射或膜渗透将分析物响应性染料装载到细胞中，可选地耦合到载体颗粒上。然后，细胞内分析物浓度将通过多路读出染料信号来确定。为了避免与染料潜在的(这里是不需要的)pH响应的串扰，染料的分析物响应将以时间分辨荧光显微镜(FLIM)或表面增强拉曼散射(SERS)显微镜的形式被横向读出。在二维和三维共培养系统中，通过用SERS标签标记细胞将促进不同细胞中染料的多路读出。

Sensing of intracellular analytes (mostly located in endosomes/lysosomes) will be performed by loading cells with analyte-responsive dyes, optionally coupled to carrier particles, via endocytosis, microinjection, or membrane permeation. Intracellular analyte concentrations will then be determined by multiplexed read-out of the dye signals. In order to avoid crosstalk with the potential (here unwanted) pH-response of the dyes, the analyte-response of the dyes will be read-out laterally resolved either in the form of time-resolved fluorescence microscopy (FLIM) or surface-enhanced Raman scattering (SERS) microscopy. Multiplexed read-out of dyes in different cells within two- and three-dimensional co-culture systems will be facilitated by cell-labelling with SERS tags.