Suction pipette measurements of mammalian rod photoreceptor recovery following intense bleaching exposures

该项目的目的是发现阻止视网膜光感受器在停止暴露于极明亮的照明后立即恢复的事件和过程。记录将从哺乳动物视网膜分离的单杆光感受器细胞中进行。这项研究将揭示视紫红质吸收光线时产生的“感光产物”的相对作用：例如，变视紫质和视蛋白等中间体。所获得的分子知识将帮助我们理解为什么眼睛经历了非常强烈的光线后，视觉系统恢复得如此缓慢。

The aim of this project is to discover the events and processes that prevent retinal photoreceptors from recovering instantaneously following the cessation of exposure to extremely bright illumination. Recordings will be made from single rod photoreceptors cells isolated from the mammalian retina. The work will uncover the relative roles of the 'photoproducts' created when rhodopsin absorbs light: e.g. intermediates such as metarhodopsin and opsin. The molecular knowledge obtained will help us to understand why it is that the visual system recovers so slowly after the eye has experienced very intense light.