Generating broad and stable resistance to gemini- and nanoviruses

单链DNA双生病毒和纳米病毒被认为是对热带和亚热带作物生产的主要威胁。目前还没有针对这些病毒的有效控制策略。我们一直在开发一种新的转基因策略，以在病毒Rep蛋白存在的情况下激活自杀基因来产生对单链DNA病毒的抗性。重要的是，我们已经证明了使用烟草黄矮病巨型病毒的原理。在这个项目中，我们的目标是进一步将这一策略发展成一种广泛和稳定的机制，以产生对所有已知双生病毒和纳米病毒的抗性。

The ssDNA geminiviruses and nanoviruses are considered a major threat to crop production in the tropics and subtropics. There are currently no effective control strategies for these viruses. We have been developing a novel transgenic strategy for generating resistance to ssDNA viruses based on the activation of a suicide gene in the presence of the viral Rep protein. Importantly, we have demonstrated proof-of-principle using Tobacco yellow dwarf mastrevirus. In this project, we aim to further develop this strategy into a broad and stable mechanism for generating resistance to all known geminiviruses and nanoviruses.