Development of method for identification of whale species using transposable elements

根据存在或缺乏sin(短inteispersed重复元素)序列在基因组或网站,我们尝试检测遗传差异,使用在PCR侧面,在不同种类的鲸鱼获得的结果表明,一些孤立的位点插入的正弦序列是特定于一个家庭或总科的鲸鱼如下所示(位点的名称括号)Delphinoidea (Mago19轨迹),Iniidae (Amz11轨迹),此外，我们还分离出了Spm2、10和14，它们都具有抹香鲸特有的插入位点。通过对上述位点的单次PCR检测，我们可以清楚地识别出这些鲸群。我们分离出另外23个基因座（它们的名字没有在这里显示），这些基因座在几个鲸鱼群体中显示出一个共享的SINE序列，观察几个这样的基因座组合可以更详细地识别鲸鱼群体。目前的结果表明，从方便和准确识别鲸鱼家族的角度来看，分析SINE插入是非常有效的。它有望鉴定出可用于鉴定鲸鱼物种的正弦插入位点

Based on the presence or the absence of a SINE (Short inteispersed repetitive element) sequence at or sites in a genome, we attemped to detect genetic differences, using flanking PCR, among different species of whales The obtained results showed that some of the isolated loci have insertion of a SINE sequence which is specific to one of the families or superfamilies of whales as follows (the names of the loci were shown in parentheses) Delphinoidea (the locus Mago19), Iniidae (the locus Amz11), Ziphiidae (the loci Tuti24, 28, and 35), Physeteridae (the loci Spm4, 9, 13, 15, and 49), and Balaenopteridae (the Loci Hump20 and 203) In addition to these, we also isolated Spm2, 10, and 14, all of which exhibit insertions unique to the sperm whale By a single PCR experiment for detection of the loci above, it is possible to identify those groups of whales clearly Moreover, we isolated other 23 loci (their names are not shown here) that exhibited sharing of a SINE sequence among several groups of the whales Observation of several such loci in combination makes it possible to identify groups of the whales in more detail The present results suggest, from the view point of convenient and accurate identification of whale families, that analysis of SINE insertion is quite efficient In the future, it is expected to become possible to identify SINE-inserted loci that can be used foi identification of species of whales