Effects of collagen fibers on the reorganization of samll intestine, especially on the formation of villi from fetal mouse small intestinal cells in cultures.

我们观察了胎儿小肠细胞培养绒毛的形成过程，并观察了胶原纤维在重组过程中的作用，胶原纤维是细胞外基质的组成成分之一。胶原蛋白含量在培养5 ~ 7 d时达到最高水平。此时，重组小肠细胞表现出最具器官样结构。培养7天后胶原蛋白含量下降。当我们加入一种胶原合成抑制剂l -azethidine-2-羧酸（AzC）时，上皮细胞没有在细胞团块的表层形成。胶原蛋白纤维排列不规律。证实了除了胶原纤维的定量变化外，胶原纤维的规则排列对器官的重组和维持也很重要。由于胶原纤维对重组的重要性得到证实，我们检查了与胶原生成细胞增殖相关的成纤维细胞生长因子（FGF）和成纤维细胞生长因子受体（FGFR）的mnrna的出现。孵育2 ~ 3天后，FGF-1和FGF-2的外观存在一定差异。重组与这些生长因子的关系有待进一步研究。本实验采用的培养方法可以观察到胶原纤维在小肠器官发生过程中的作用。该培养体系可为今后阐明胶原纤维在伤口愈合和器官发生过程中的作用提供有用的工具。

We observed the process of the formation of villi in cultures of fetal small intestinal cells, and the role of collagen fibers, which are one of the components of extra-cellular matrix, was also observed during reorganization. Collagen content reached the highest level at 5 to 7 days of incubation. At this time, the reorganized small intestinal cells showed the most organ-like structures. Collagen content decreasedafter 7 days of incubation.When we added L-azethidine-2-carboxylic acid (AzC)that is an inhibitor of collagen synthesis, the formation of epithelium did not occur on the surface layer of cellular mass. Collagen fibers did not take a regular arrangement. It was confirmed that the regular arrangement of collagen fibers was also important for the reorganization and maintenance of organs, in addition to the quantitative changes of collagen fibers. Because the importance of collagen fibers for reorganization was confirmed, we examined an appearance of mnRNAs of bibroblast growth factor(FGF) and fibroblast growth factoar receptor (FGFR) related to proliferation of collagen producing cells. After 2 to 3 days of incubation, there were some differences between the appearance of FGF-1 and FGF-2. Further investIgation is necessary for the relation of reorganization and these growth factors.This culture method used here can observe the role of collagen fibers during organogenesis of small intestine. This culture system may provide a useful tooL for elucidation of role of collagen fibers in a process of the wound healing and organogenesis in future.