A study on the possibility of gastric cancer caused by Helicobacter pylori using human normal epithelial cells in vitro.

世界卫生组织国际癌症研究机构的一个工作组在1994年进行的研究表明，幽门螺杆菌是人类的第一组致癌物质。流行病学研究表明，幽门螺杆菌感染与胃癌有关。但到目前为止，在实验中几乎没有直接的证据。我们在体外研究了胃癌和幽门螺杆菌之间的关系。我们建立了人正常胃粘膜上皮细胞系。本研究以胃癌细胞株（KATO-3）和其他细胞株为材料，探讨了幽门螺杆菌是否引起染色体改变和细胞凋亡，以及幽门螺杆菌与胃癌和端粒酶的关系。H.pylori培养上清可引起Mm-2 T细胞染色体数目的改变和Mm-2 T等细胞的形态学改变，NH_3和NH_2CL可诱导细胞凋亡，部分H.pylori培养上清可诱导细胞凋亡。端粒酶活性为20%。这些数据表明胃癌和幽门螺杆菌之间的关联。

A working Group of the World Health Organization International Agency for research or cancer conducted in1994 that H.pylori is a group 1 carcinogen in humans. Epidemiological studies have indicated H.pylori infection is associated with gastric cancer. But until now there is few direct proof in experiments. We examined the association between gastric cancer and H.pylori in vitro. We established epitherial cell line derived from human normal gastric mucosa. Using these cells, gastric cancer cell line(KATO-3) and other cell lines, we examined whether H.pylori induce the change of chromosome and apoptosis and the association among cancer, H.pylori and teromerase. H.pylori culture supernatant induced the change of chromosome number in Mm-2T cells and morphological changes in Mm-2T and other cell lines .NH_3 and NH_2CL induced apoptosis and among some H.pylori culture supematant induced apoptosis. Teromerase activity was 20% in intestinalmetaplasia. These data indicate the association between gastric cancer and H.pylori.