Effects of Nitric Oxide Synthase Inhibitor on Organ Blood Flow Distribution during Hemorrhagic Shock

1、探讨一氧化氮合酶（NOS）抑制剂对失血性休克脏器血流分布的影响。在麻醉的狗中，通过将血液从循环中引流到储血器中，并通过进一步取血或输血维持90毫米的低血压（平均动脉压=4OmmHg）。在90 mm休克期后，给予10mg/kg n -硝基- l -精氨酸甲酯（L-NAME组：n=8）或生理盐水（对照组：n=8）。采用放射性微球法测定两组患者降压前及L-NAME或生理盐水给药后5mm器官血流分布。两组间器官血流分布无明显差异。在两组中均观察到出血性休克时脑和心脏血流分布的生理变化（集中）。（Circulation Control 18; 541-547: 1997）2、我们还研究了出血性休克时血浆肾上腺髓质素（一种新型血管舒张肽）浓度的变化。在麻醉的狗中，通过将血液从循环中引流到储血器中，并通过进一步取血或输血维持90毫米的低血压（平均动脉压=4OmmHg）。出血犬（n=8）血浆肾上腺髓质素浓度明显高于假手术犬（n=8）。（麻醉与镇痛88；326-328;1999）

1, We investigated the effects of nitric oxide synthase (NOS) inhibitor on organ blood flow distribution in hemorrhagic shock. In anesthetized dogs, hypotension (mean arterial pressure=4OmmHg) was achieved by draining blood from their circulation into a reservoir and maintained for 90 mm by further removing or transfusing blood. Following the 90 mm shock period, 10mg/kg N-nitro-L-arginine methyl ester (L-NAME group : n=8) or saline (control group : n=8) was administered. Organ blood flow distribution was measured before hypotension and I 5mm after L-NAME or saline administration in both groups by the radioactive microsphere method. There was no signicant difference in organ blood flow distribution between the two groups. A physiological shift in blood flow distribution during hemorrhagic shock to the brain and heart (centralization) was observed in both groups. (Circulation Control 1 8 ; 541-547 : 1997)2, We also investigated the change of plasma concentration of adrenomedullin, a novel vasodilating peptide, in hemorrhgic shock. In anesthetized dogs, hypotension (mean arterial pressure=4OmmHg) was achieved by draining blood from their circulation into a reservoir and maintained for 90 mm by further removing or transfusing blood. Plasma concentration of adrenomedullin was increased in hemorrhagic dogs (n=8) more significantly than in sham dogs (n=8). (Anesthesia & Analgesia 88 ; 326-328 : 1999)