Assessment of ovarian fecundity in terms of telomere length and telomerase activity.

许多端粒酶活性的研究已被用于临床诊断和治疗癌症。对具有高增殖活性的生殖细胞的研究很少。本研究探讨了端粒酶活性与卵子和胚胎发育的关系。在人体外受精- et实验中，每个注射器分别抽吸每个卵泡，从每个卵泡液中获得壁粒细胞和积云粒细胞。用TRAP法测定两种颗粒细胞的端粒酶活性。用小鼠卵母细胞检测卵母细胞端粒酶活性，用TRAP法检测卵母细胞和胚胎发育过程中端粒酶活性的变化。端粒酶活性在积云细胞和壁粒细胞中均有检测。壁粒细胞活性高于积云粒细胞活性。端粒酶活性也存在于卵母细胞和胚胎的不同阶段。在卵母细胞阶段，PMS注射后24h的卵母细胞高于PMS注射后48h、hCG注射后4、8 h和刚排卵后的卵母细胞。胚胎期端粒酶活性在1期、2期和molura期较低，在囊胚期活性开始升高。因此，端粒酶的活性在卵母细胞和胚胎的发育过程中是波动的，可能对卵母细胞和胚胎的发育有影响。

Many studies of telomerase activity has been performed for the clinical application to diagnosis and treatment of cancer. Few studies have been performed about the reproductive cells which have highly proliferative activity. In this study, the relationship between telomerase activity and the development of eggs and embryos was examined.In IVF-ET of human, each follicle was aspirated separately by each syringe and mural granulosa cells and cumulus granulosa cells were obtained from each follicular fluid. Then telomerase activity of both kinds of granulosa cells were measured by TRAP assay. And the telomerase activity of oocyte was measured using mouse oocytes and the fluctuation of the telomerase activity during the developmental stage of oocyte and embryo was examined by TRAP assay.Telomerase activity was detected in both cumulus and mural granulosa cells. And the activity of mural granulosa cells was higher than that of cumulus granulosa cells. Telomerase activity was also present in both oocytes and embryos at various stages. In the oocyte stages, the oocyte 24hrs after PMS injection showed the higher than that of the oocytes 48 hrs after PMS, 4 and 8 hrs after hCG and just after ovulation. In the embryo stages, the telomerase activity was low at one, two and molura stage and the activity started to increase at blastocyst stage.Thus the activity of telomerase is fluctuating during the development of oocyte and embryo, and might have effects of the development of oocyte and embryo.