The allosteric effect in hemoglobin.

1)研究了用异常血红蛋白和化学修饰的血红蛋白改变脱氧血红蛋白分子的氧亲和力时，分子各部分的构象变化。结果发现，血红素和珠蛋白部分的构象变化不是以一致的方式发生的，与两态变构模型的预期相反。2)发现四种金属原卟啉对脱氧血红素有模型化作用。其中，对镍原卟啉IX的研究最为广泛。从Ni-Fe杂化血红蛋白的氧平衡性质来看。镍原卟啉IX不仅在未修饰的血红蛋白中，而且在修饰后的血红蛋白中，其行为就像“冷冻的”脱氧血红素。3)用镍原卟啉制备的血红蛋白氧化中间态模型为构象态，不能在两态变构模型的框架内描述。4)用铁系过渡金属离子取代铁，研究了铁的电子态与球蛋白的相互作用。具有金属原卟啉的血红蛋白的构象状态根据元素在周期表中的位置而改变。对这一结果还没有给出完整的解释。5)获得的数据表明了11交互作用的重要性。6)89组氨酸对碱性玻尔效应的贡献约为25%。7)通过对大肠杆菌进行基因操作，有可能制备出具有任何一级结构的血红蛋白。8)搭建了一套测量紫外光激发下的共振喇曼光谱的装置。9)通过构象能函数的计算机计算，分析了肌红蛋白的动态三维结构。

1) The conformational changes of various parts ot a deoxyhemoglobin molecule were investigated when its oxygen affinity was altered by use of abnormal hemoglobins and chemically modified ones. It was found that the conformational changes around heme and those of the globin moiety did not take place in concerted manner on the contrary to the expectation of the two state allosteric model. 2) Four metalloprotoporphyrins were found to medel the deoxyheme. Among them Ni-protoporphyrinIX has been studied most extensively. Judging from the oxygen equilibrium properties of Ni-Fe hybrid hemoglobins. Ni-protoporphyrinIX behaves just like "frozen" deoxyheme not only in unmodified hemoglobins but also in modified ones. 3) The model for the intermediate state of the oxygenation of hemoglobin prepared by use of Ni-protoporphyrin showed a conformational state, which could not be accomodated in the framework of the two state allosteric model. 4) The interaction between the electronic state of iron and the globin moiety was investigated by substituteing iron serries transition metal ions for iron. The conformational state of the hemoglobin having the metalloprotoporphyrin changed according to the position of the element in the periodical table. No complete explanation of the result has been given. 5) Data were obtained to suggest the importance of the 1 1 interaction. 6) 89 Histidine was shown to contribute about 25% of the alkaline Bohr effect. 7) It became possible to prepare a hemoglobin having any primary structure by gene manipulation of E. coli. 8) An equipment to measure a resonance Raman spectra excited by ultraviolet laser beams was constructed. 9) The dynamic three dimentional structure of myoglobin was analyed by the computer calculation of the conformational energy function.