Study on the mechanism of immobilized enzyme activity and clinical application

制备抗血栓形成材料的方法之一是将纤溶酶固定在载体表面上。本课题主要研究固定化酶活性的作用机制及临床应用。实验材料以组织培养-尿激酶和组织-纤溶酶原激活剂为酶，尼龙和聚氨酯为载体。通过肽连接程序进行固定。结果如下：固定化酶在贮藏6个月后仍保持较高的纤溶活性。即使对抑制剂和抗体，活性也相对稳定。酶固定化材料在体外和体内均具有有效的抗血栓形成能力。但随着置管时间的延长，纤溶活性逐渐下降。现在，这些抗血栓形成导管已用于IVH和TPN的临床病例的调查。

One of the methods of preparing an antithrombogenic material is to immobilize a fibrinolytic enzyme on the surface of a carrier.In this project, we studied the mechanisms of immobilized enzyme activity and clinical applications.The materials used in this experiment were tissue-culture-urokinase and tissue-plasminogen activator as enzyme, and nylon and polyurethane as carrier. Immobilization was performed by peptide linking procedure.These results were as follow1. Immobilized enzymes maintained the high fibrinolytic activity after 6 months storage.2. The activities were relatively stable even against the inhibitor and antibody.3. Enzyme immobilized materials had the effective antithrombogenic ability in vitro and in vivo.4. But, the fibrinolytic activity decreased gradually after a prolonged catheterization period.5. Now, these antithrombogenic catheters have been utilized in clinical cases with IVH and TPN for the Investigations.