A study on the role of humoral antibody against Bacteroides gingivalis.

牙龈拟杆菌与许多成人牙周炎有关。对B的体液免疫应答升高。在成人牙周炎的受试者牙龈炎已在我们的实验室报告。在本研究中，我们研究了杀菌活性与B。检测人血清中牙龈炎菌的含量及IgG抗体水平，B.在厌氧条件下检测牙龈炎菌株对补体介导的人血清杀菌活性的影响。血清中含有高浓度的IgG抗体的微生物增强杀菌活性。在不含特异性抗体的血清或完整细胞吸收的血清中未发现增强。B对混合血清杀伤的敏感性不同。牙龈菌株。B的细胞。牙龈炎不仅通过经典途径而且通过替代途径激活汇集的人血清补体。结果表明，B.我们报道了龈沟液中含有大量的含有抗B IgG抗体的免疫球蛋白。牙龈炎和补体系统。这些发现可能支持这样的论点，即在牙龈缝隙中的特异性抗体和补体是体内宿主抵抗微生物的重要决定因素。

Bacteroide gingivalis has been implicated in many cases of adult periodontitis. Elevation of humoral immune responses to B. gingivalis in subjects with adult periodontitis has been reported in our laboratory. In the present study, we investigated the relationship between bactericidal activity for B. gingivalis in human serum and IgG antibody levels to the organisms.Susceptibility of B. gingivalis strains to the complement mediated bactericidal activity of human serum was examined under anaerobic conditions. Serum containing high concentrations of IgG antibody to the microorganisms enhanced the bactericidal activity. No enhancement was found in serum which did not contain the specific antibody or in serum absorbed with intact cells. The sensitivity to the killing by pooled serum differed among B. gingivalis strains. Cells of B. gingivalis activated the pooled human serum complement not only through the classical pathway but also through an alternative pathway. It was found that the susceptibility of B. gingivalis to the bactericidal activity was classical pathway-dependent.We reported that gingival crevicular fluid contained both appreciable amounts of immunoglobulins containing IgG antibody to B. gingivalis and a complement system. These findings may support the argument that the specific antibody and complement in the gingival crevice are important determinants of host resistance to the micro-organism in vivo.