PHYSIOLOGICAL ROLE OF SHORT-CHAIN FATTY ACIDS IN CONTROL OF PANCREATIC EXOCRINE SECRETION OF RUMINANTS.

据报道，短链脂肪酸刺激绵羊胰腺外分泌。然而，尚不清楚这种作用是否在控制胰腺分泌中起生理作用。本研究旨在阐明绵羊生理条件下胰腺外分泌对短链脂肪酸的反应。1.采用头-气-空气相色谱法对血液中的短链脂肪酸进行了分析。2.饲喂2h后血浆醋酸酯浓度为2458<±bb0 149unol/1。3.以12.5、25、50和75 <微> mol/kg的速率静脉滴注乙酸钠（pH:7.4）。尽管在各给药组中，输注期间血浆中乳酸盐浓度均低于饲喂后，但min可增加胰腺外分泌的反应。4.胰腺分泌的反应随着短链脂肪酸碳数的增加而增强。5.cck-8经静脉滴注醋酸酯后胰腺反应小于未滴注cck-8的胰腺反应。相比之下，醋酸酯输注组对分泌素的反应大于未输注组。6.静脉注射丁酸酯刺激后，胰腺外分泌明显受到抑制。7.胰液中HCO_3的分泌作用为分泌素> CCK-8 <相似的>丁酸盐。8.短链脂肪酸在不同部位（腹腔、十二指肠或静脉）的应用导致胰腺外分泌的不同反应。

IT IS REPORTED THAT THE SHORT-CHAIN FATTY ACIDS STIMULATE THE PANCREATIC EXOCRINE SECRETION IN SHEEP. HOWEVER, IT IS UNCLEAR WHETHER THIS EFFECT PLAYS PHYSIOLOGICAL ROLE IN CONTROL OF PANCREATIC SECRETION. THE PRESENT STUDY WAS UNDERTAKEN TO CLARIFY THE RESPONSE OF PANCREATIC EXOCRINE SECRETION TO SHORTCHAIN FATTY ACIDS UNDER THE PHYSIOLOGICAL CONDITION IN SHEEP. 1.SHORT-CHAIN FATTY ACIDS IN BLOOD WERE ANALYZED BY THE HEAD-GAS-SPACE GAS CHROMATOGRAPHY. 2.THE CONCENTRATION OF ACETATE IN PLASMA 2hrs AFTER FEEDING WAS 2458<plus-minus>149unol/1. 3.INTRAVENOUS INFUSION OF Na-ACETATE(pH:7.4) AT THE RATE OF 12.5, 25, 50 AND 75 <micro> mol/kg.min RESULTED IN INCREASED RESPONSE OF PANCREATIC EXOCRINE SECRETION, THOUGH THE ACTETATE CONCENTRATION IN PLASMA DURING INFUSION PERIOD WAS LOWER THAN THE VALUE AFTER FEEDING IN EVERY ADMINISTRATION RATES. 4.THE RESPONSE OF PANCREATIC SECRETION WAS INCREASED AS INCREASING NUMBER OF CARBON OF SHORT-CHAIN FATTY ACID. 5.THE PANCREATIC RESPONSE TO THE INTRAVENOUS ADMINISTRATION OF CCK-8 WITH INTRAVENOUS INFUSION OF ACETATE WAS LESS THAN THAT WITHOUT INFUSION. IN CONTRAST, THE RESPONSE TO SECRETIN WAS BIGGER WITH ACETATE INFUSION THAN WITHOUT ONE. 6.A MARKED INHIBITION OF PANCREATIC EXOCRINE SECRETION APPEARED AFTER THE STIMULATION BY INTRAVENOUS INJECTION OF BUTYRATE. 7.THE SECRETORY EFFECT OF HCO_3 INTO PANCREATIC JUICE WAS SECRETIN > CCK-8 <similar equal> BUTYRATE. 8.THE ADMINISTRATION OF SHORT-CHAIN FATTY ACIDS INTO THE DIFFERENT SITE, INTRARUMINALLY, INTRADUODENALLY OR INTRAVENOUSLY RESULTED IN DIFFERENT RESPONSES OF PANCREATIC EXOCRINE SECRETION.