Ceramic dendritic fibrous nanosilica (DFNS) structures for continuous lytic bioprocesses

该项目的目的是开发一种使用树枝状纤维状纳米二氧化硅(DFNS)颗粒的模块化连续流动模块，用于有效的细胞裂解。细胞裂解是细胞灭活或提取胞内产物生物处理的关键步骤。诸如产品回收、下游工艺挑战和产品质量等因素受到细胞破坏类型的严重影响。目前的标准通常非常耗费成本、耗时，而且不能连续适用。在该项目的前期工作中，已经成功地制备了DFNS纳米颗粒，并在陶瓷表面涂覆了DFNS结构。使用这些颗粒，DFNS结构的明显机械溶解效应已经在静态和动态初步试验中被证明。一个动态裂解系统的原型在五代后实现了细胞的完全裂解。这突出了该项目在开发具有成本效益、高效、模块适用和可持续操作的裂解模块方面的巨大潜力。在这个项目中，将利用先进陶瓷(先进陶瓷不来梅)和生物工艺工程(技术化学研究所)领域的专业知识，以跨学科的方式成功合作。除了制备和优化用于原核和真核细胞机械裂解的精细DFNS结构外，还将利用DFNS独特的纤维形态通过固定化酶来实现协同裂解效果。由于DFNS的结构很容易修改，该项目除了优化细胞裂解外，还提供了进一步的长期机会。疏水官能化和缺氧表面修饰可用于获得催化或光催化性能。

The aim of this project is to develop a modular continuous flow module with dendritic fibrous nanosilica (DFNS) particles for effective cell lysis. Cell lysis is the key process step in bioprocessing for cell inactivation or extracting intracellular products. Factors such as product recovery, downstream process challenges, and product quality are critically influenced by the type of cell disruption. Current standards are usually very cost-intensive, time-consuming and cannot be applied continuously. In preliminary work for this project, DFNS nanoparticles were already successfully produced and ceramic surfaces were coated with DFNS structures. Using these particles, a clear mechanical lysis effect of the DFNS structures could already be demonstrated in static and dynamic preliminary tests. A prototype of a dynamic lysis system achieved complete cell lysis after five passages. That highlights the great potential of this project to develop a cost-effective, efficient, modularly applicable and continuously operable lysis module. In this project, the expertise in the field of advanced ceramics (Advanced Ceramics Bremen) and bioprocess engineering (Institute of Technical Chemistry) will be used to successfully collaborate in an interdisciplinary manner. In addition to the fabrication and optimization of the delicate DFNS structures for the mechanical lysis of prokaryotic and eukaryotic cells, the unique fibrous morphology of DFNS will be used to achieve a synergistic lysis effect by means of enzyme immobilization. Since the DFNS structures are easily modifiable, this project offers further long-term opportunities in addition to optimized cell lysis. Hydrophobic functionalizations and oxygen-deficient surface modifications could be used to achieve catalytic or photocatalytic properties.