Transport of Phosphoenolpyruvate across the Biological Membrane

本项目研究磷酸烯醇式丙酮酸通过红细胞膜的转运机制。本研究的主要成果如下：1.磷酸烯醇式丙酮酸的转运是由红细胞膜带3蛋白介导的，也是氯离子和磷酸盐的转运。2.带3的COOH末端的第60位赖氨酸残基构成转运系统活性中心的至少一部分。3.除赖氨酸残基外，带3内的组氨酸残基参与转运系统。组氨酸位于细胞膜的细胞内表面，并与转移位点发生变构相互作用。4.磷酸烯醇式丙酮酸不仅可以穿过红细胞膜，还可以穿过肾脏和肝脏的细胞膜。

This project is concerned with the transport mechanisms of phosphoenolpyruvate across the red cell membrane. Results of this project are summarized as follows: 1. Transport of phosphoenolpyruvate is mediated by Band 3 protein of red cell membrane as well as the transports of chloride and phosphate. 2. The 60th-lysine residue from the COOH-terminal end of Band 3 constitutes at least a part of the active center of the transport system. 3. In addition to the lysine residue, a histidine residue within Band 3 participates in the transport system. The histidine is located at the intracellular surface of the cell membrane and allosterically interacts with the transfer site. 4. Phosphoenolpyruvate transports across not only the red cell membrane but also the cell membranes of kidney and liver.